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生物来源
mouse
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
1B10, monoclonal
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~ 1 mg/mL
技术
immunofluorescence: 5-10 μg/mL using Human foreskin fibroblast Hs68 cells.
同位素/亚型
IgM
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... S100A4(6275)
相关类别
一般描述
A surface protein characterizing human fibroblasts and fibroblast cell lines, as well as tissue macrophages and 95% of the peripheral blood monocytes. This fibroblast protein antigen has also been demonstrated on malignant fibrosarcoma tissue, human synovial, foreskin and thymic fibroblasts and it is absent from human epithelial cells and lymphocytes.
特异性
Monoclonal Anti-Human Fibroblast Surface Protein specifically recognizes human fibroblast cells, tissue macrophages and peripheral monocytes .Due to its specific reaction against human fibroblasts, the antibody is useful for removal of fibroblasts from human cultured cells either by inhibition of fibroblasts adherence to the culture vessel or by cytotoxic effect in the presence of rabbit complement.
免疫原
Cultured human thymic fibroblasts
应用
The antibody is recommended for use in various immunological techniques including Immunofluorescence, flow cytometry, immunohistochemistry and immunoblotting.
生化/生理作用
Overgrowth of epithelial cells by fibroblasts has long been an obstacle to cultivation of normal and abnormal epithelial cells.Characterization and identification of cells is important for studying functional significance, correlation of cultures with tissue origin, identification of the lineage to which the cells belongs to or identifying the precursor status of the cells.
外形
Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.
储存及稳定性
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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