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Merck
CN

SAB4200805

Sigma-Aldrich

Anti-β-Galactosidase antibody, Mouse monoclonal

clone GAL-13, purified from hybridoma cell culture

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别名:
Anti-Beta-gal, Anti-Beta-galactosidase, Anti-Lactase
UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

GAL-13, monoclonal

描述

Research area: Microbiome

形式

buffered aqueous solution

种属反应性

E. coli

包装

antibody small pack of 25 μL

浓度

~1.0 mg/mL

技术

immunofluorescence: suitable
immunohistochemistry: suitable
indirect ELISA: 0.5-1 μg/mL using anti-Mouse antibody for coating, primary Anti-Beta-GAL antibody followed by addition of β-d-galactosidase enzyme from E. coli and 2-Nitrophenyl β-D-galactopyranoside as substrate.

同位素/亚型

IgG1

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

Escherichia coli K12 ... Lacz(945006)

一般描述

Anti-nitric oxide synthase, inducible antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the NOS-IN hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a synthetic peptide corresponding to the C terminal region of nitric oxide synthase (NOS) of mouse macrophage origin (iNOS, also termed macNOS), conjugated to KLH
Beta galactosidases have been obtained from microorganisms such as fungi, bacteria and yeasts; plants, animals cells and from recombinant sources. This enzyme has two main applications; the removal of lactose from milk products for lactose intolerant people and the production of galactosylated products.Monoclonal Anti-Beta-Galactosidase recognizes soluble Beta-d-galactosidase and retains the enzymatic activity. The antibody reacts with the native enzyme and does not recognize denatured or reduced Beta-galactosidase. The Anti-Beta-Galactosidase antibody maybe useful tool for immunoenzymatic staining of blood and bone marrow smears or tissue sections. In addition, this product may be used as a primary antibody for the detection and purification of recombinant fusion hybrid proteins, which contain Beta-galactosidase (lacZ) in the cloning vector.

免疫原

Beta-D-Galactosidase purified from E. coli

应用

Anti-Nitric Oxide Synthase, Inducible antibody, Mouse monoclonal has been used in: immunoblotting immunofluorescence fluorescence-activated cell sorting 3 (FACS3) immunohistochemistry
The antibody is recommended to use in various immunological techniques, including ELISA, Immunofluorescence and immunohistology.

生化/生理作用

NO is a messenger molecule mediating diverse functions including vasodilatation, neurotransmission, antimicrobial and anti-tumor activities. It is implicated as a pathogenic mediator in a variety of conditions, including central nervous system (CNS) disease states, such as the animal model of multiple sclerosis (MS) and experimental allergic encephalomyelitis.

外形

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

其他说明

This product is for R&D use only, not for drug, household, or other uses.

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Nitric oxide release: Part II
Carpenter AW and Mark HS
Chemical Society Reviews, 41(10), 3742-3752 (2012)
Anjali Bajpai et al.
Developmental biology, 457(1), 1-8 (2019-09-27)
The Hedgehog (Hh) morphogen regulates growth and patterning. Since Hh signaling is also implicated in carcinogenesis, it is conceivable that de novo Hh-secreting organizers, if formed in association with oncogenic hit could be tumor-cooperative. Here we validate this hypothesis using

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