生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
MB-11, monoclonal
形式
buffered aqueous solution
种属反应性
human
浓度
~1.0 mg/mL
技术
ELISA: 0.1-0.2 μg/mL using 2.5 μg/mL Human IgM myeloma for coating
immunoblotting: suitable
同位素/亚型
IgG2b
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgM protein.
Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocyte membranes. IgM is suggested to act as the first line of defense during microbial infections. IgM has a pentameric structure, in which monomers are linking together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.
特异性
Anti-Human IgM (m-chain specific) antibody, Mouse monoclonal specifically recognizes m chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, k, l, and g chains.
免疫原
Purified human IgM
应用
The antibody is recommended to use in various immunological techniques, including ELISA and Immunoblot.
生化/生理作用
Immunoglobulin M (IgM) acts as an antigen specific part of the B cell antigen receptor on the surface of B lymphocytes that are not stimulated, in its monomeric form. Polymeric IgM molecules also serve as important activators of the classical complement cascade. IgM is essential in agglutination and cytolytic reactions. IgM serves as the antigen receptor of naive B cells and is involved in B cell maturation and complement activation.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as a preservative.
其他说明
This product is for R&D use only, not for drug, household, or other uses.
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WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
G Vidarsson et al.
Infection and immunity, 66(6), 2866-2870 (1998-05-29)
Streptococcus pneumoniae is a major respiratory pathogen of infants, children, and the elderly. Polysaccharide vaccines have been useful in adult populations but do not elicit protective immunity in infants and young children. To enhance their immunogenicity, vaccines of pneumococcal polysaccharides
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