Anti-Glycoprotein (MDR) antibody, Mouse monoclonal

Anti-P-Glycoprotein (MDR) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma F4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multidrug resistance protein 1 (MDR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters superfamily. It is ubiquitously expressed in kidneys, intestines, placenta, liver, adrenal glands blood-brain barrier (BBB), placenta and testis.

Anti-P-Glycoprotein (MDR) antibody, Mouse monoclonal recognizes an epitope located at the N-terminal region of P-glycoprotein (Pgp), at the third extracellular loop of the molecule, also known as ABCB1 protein. The antibody specifically detects MDR1 P-glycoprotein, but does not recognize MDR3. Anti-P-Glycoprotein (MDR) cross reacts with MDR1 P-glycoprotein from human and hamster origin.

a mixture of human and hamster drug-resistant whole cells and crude plasma membranes.

Anti-Glycoprotein (MDR) antibody, mouse monoclonal may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry
• immunohistochemistry
• cellular enzyme-linked immunosorbent assay (ELISA)
• flow cytometry (FACS)
• cell surface radioimmunoassay (RIA)

Multidrug resistance protein 1 (MDR1) functions as an adenosine triphosphate (ATP)-dependent efflux pump. It is also involved in extruding certain xenobiotics and protect cells from toxicants. MDR1 gene is overexpressed in different cancer types including leukemias, lymphomas, myelomas, breast cancer, ovarian cancer, gastrointestinal stromal tumor (GIST), non-small cell lung cancer (NSCLC), fallopian tube, colon, renal, and thyroid cancers. Overexpression of MDR1 gene plays an important role in prompting multidrug resistance in cancer chemotherapy causing high resistance of cancerous cells to a wide variety of substrate anticancer drugs.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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