SAB4200767
Anti-Cathepsin D antibody, Mouse monoclonal
clone CTD-19, purified from hybridoma cell culture
别名:
Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CTD-19, monoclonal
Application:
immunoblotting
immunofluorescence
immunohistochemistry
immunofluorescence
immunohistochemistry
Species reactivity:
human, rabbit
Citations:
5
Technique(s):
immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
Uniprot accession no.:
产品名称
Anti-Cathepsin D antibody, Mouse monoclonal, clone CTD-19, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CTD-19, monoclonal
form
buffered aqueous solution
species reactivity
human, rabbit
concentration
~1.0 mg/mL
technique(s)
immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CTSD(1509)
Application
Anti-Cathepsin D antibody, Mouse monoclonal has been used in:
- immunoblotting
- immunohistochemistry
- immunofluorescence
Biochem/physiol Actions
CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane.
General description
Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain.
Immunogen
Cathepsin D Purified from human liver
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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flash_point_c
Not applicable
存储类别
10 - Combustible liquids
flash_point_f
Not applicable
法规信息
常规特殊物品
此项目有
Veronika Stoka et al.
Ageing research reviews, 32, 22-37 (2016-04-30)
Lysosomes and lysosomal hydrolases, including the cathepsins, have been shown to change their properties with aging brain a long time ago, although their function was not really understood. The first biochemical and clinical studies were followed by a major expansion
Cathepsin D as a therapeutic target in Alzheimer's disease.
Di Domenico F, et al.
Expert Opinion on Therapeutic Targets, 20(12), 1393-1395 (2016)
The role of cathepsin D in the pathogenesis of human neurodegenerative disorders
Vidoni C, et al.
Medicinal Research Reviews, 36(5), 845-870 (2016)
Nuclear cathepsin D enhances TRPS1 transcriptional repressor function to regulate cell cycle progression and transformation in human breast cancer cells
Bach AS, et al.
Oncotarget, 6(29), 28084-28084 (2015)
Clivia Lisowski et al.
Autophagy, 18(8), 1785-1800 (2021-11-17)
Modulation of the host cell cycle has emerged as a common theme among the pathways regulated by bacterial pathogens, arguably to promote host cell colonization. However, in most cases the exact benefit ensuing from such interference to the infection process
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