产品名称
Anti-Dystrophin antibody, Mouse monoclonal, clone MANDRA1, purified from hybridoma cell culture
biological source
mouse
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
MANDRA1, monoclonal
form
buffered aqueous solution
mol wt
~427 kDa
species reactivity
zebrafish, rat, mouse, human
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunoblotting: suitable
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using acetone fixed rat tongue frozen sections
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DMD(1756)
Application
Anti-Dystrophin antibody, Mouse monoclonal has been used in:
- immunohistochemistry
- immunoblotting
- immunofluorescence
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Anti-Dystrophin antibody, Mouse monoclonal specifically recognizes an epitope located on the 128 amino acids at the end of the C-terminal domain of the human Dystrophin molecule (residues 3558-3684). The antibody reacts with Dystrophin from human, mouse, rat and zebrafish origin. The antibody is specific to dystrophin and does not react with -actinin or utrophin.
Dystrophin is absent, reduced or altered because of mutation in Duchenne and Becker muscular dystrophies (DMD/BMD) or in its homologue in mouse. Severe DMD is associated with a marked Dystrophin deficiency whereas patients with the milder form of BMD show less pronounced abnormalities of protein expression.
Disclaimer
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Dystrophin antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the MANDRA1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Dystrophin is a rod-shaped cytoskeletal protein located to the periphery (plasma membrane) of normal striated muscle fibers.
Dystrophin is encoded by the gene mapped to human chromosome Xp21.2-p21.1.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Davide Rovina et al.
Stem cell research, 40, 101544-101544 (2019-08-30)
Duchenne's muscular dystrophy (DMD) is a neuromuscular disorder affecting skeletal and cardiac muscle function, caused by mutations in the dystrophin (DMD) gene. Dermal fibroblasts, isolated from a DMD patient with a reported deletion of exons 51 to 53 in the
Analysis of dystrophin gene deletions by multiplex PCR in Moroccan patients
Sbiti A, et al.
BioMed Research International, 2(3), 158-160 (2002)
Duchenne muscular dystrophy and dystrophin: pathogenesis and opportunities for treatment
Nowak KJ and Davies KE
EMBO Reports, 5(9), 872-876 (2004)
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