生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
GS-2C4, monoclonal
形式
buffered aqueous solution
分子量
~85 kDa
种属反应性
rabbit, canine, human, mouse, monkey
浓度
~1.0 mg/mL
技术
immunoblotting: 1-2.5 μg/mL using MDCK cell line extract
immunofluorescence: suitable
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human pancreas sections
immunoprecipitation (IP): suitable
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... GSN(2934)
一般描述
Anti-Gelsolin Antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GS-2C4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Gelsolin operates in at least two variant forms encoded by a single gene and is located both intracellular (in cytosol and mitochondria) and extracellular (in blood plasma).
免疫原
Human plasma gelsolin
应用
Anti-Gelsolin antibody, Mouse Monoclonal has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
- immunoprecipitation
生化/生理作用
Gelsolin, also known as Actin-depolymerizing factor (ADF), GSN, AGEL or Brevin, is a calcium-dependent actin-binding protein. Gelsolin acts as a main modulator of actin filament formation and disassembly and is involved in a variety of cellular mechanisms including apoptosis, signal transduction, transcriptional regulation and epigenetic processes. Plasma gelsolin differs in its sequence from the cytoplasmic variant in possessing a 25-amino acid N-terminal extension. The main function of the cytoplasmic gelsolin is to remodel the actin cytoskeleton. Plasma gelsolin′s main role is to scan for actin fibrils that are released by injured tissue into the bloodstream and to bind them in a scavenger mode preventing actin from increasing the blood viscosity. Gelsolin levels correlate with inflammation and several diseases and pathologies, including cancer, infection, cardiac injury, pulmonary diseases, Alzheimer′s disease and aging thus making it a potential biomarker of diagnostic and prognostic values.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Proteomic differences between hepatocellular carcinoma and nontumorous liver tissue investigated by a combined gel-based and label-free quantitative proteomics study
Megger DA, et al.
Molecular and Cellular Proteomics, 12(7), 2006-2020 (2013)
Topological assignment of the N-terminal extension of plasma gelsolin to the gelsolin surface
Ulrike F, et al.
The Biochemical Journal, 385(3), 659-665 (2005)
Vesicular egress of non-enveloped lytic parvoviruses depends on gelsolin functioning
Bar S, et al.
PLoS Pathogens, 4(8), e1000126-e1000126 (2008)
Membrane cholesterol, lateral mobility, and the phosphatidylinositol 4, 5-bisphosphate-dependent organization of cell actin
Kwik J, et al.
Proceedings of the National Academy of Sciences of the USA, 100(24), 13964-13969 (2003)
Gelsolin amyloidosis: genetics, biochemistry, pathology and possible strategies for therapeutic intervention
Solomon JP, et al.
Critical Reviews in Biochemistry and Molecular Biology, 47(3), 282-296 (2012)
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