推荐产品
生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
GE-1, monoclonal
形式
buffered aqueous solution
种属反应性
human
浓度
~1.0 mg/mL
技术
ELISA: 0.15-0.3 μg/mL using 1 μg/mL Human IgE myeloma for coating
immunoblotting: suitable
immunoprecipitation (IP): 1-2 μg/test using Human IgE myeloma
同位素/亚型
IgG2b
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
Anti-Human IgE antibody, Mouse monoclonal (mouse IgG2b isotype) is derived from the GE-1 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgE myeloma protein. Immunoglobulin E (IgE) is the least abundant immunoglobulin isotype in circulation. IgE is produced by plasma cells and is highly functional in the lung, skin and mucus membranes. Normal levels of IgE in serum are less than 1 μg/ml.
免疫原
purified human IgE myeloma protein
应用
Anti-Human IgE antibody, Mouse monoclonal has been used in various immunological techniques, including enzyme linked immunosorbent assay (ELISA), immunoblot, immunoprecipitation and IgE quantification using piezoelectric (Pz) crystal bioassay.
生化/生理作用
Immunoglobulin E (IgE) plays a critical role in allergic reactions and host protection from parasites. Serum IgE levels are highly elevated during an allergic reaction (such as atopic eczema, extrinsic asthma or hay fever, fungal and parasitic infection, atopic disease or primary immunodeficiency). Detection and measurement of total IgE concentrations is crucial for diagnosis, monitoring and treatment of allergy patients. In addition, the recognition and isolation of allergen-specific IgE can lead to progress in understanding of pathophysiologic mechanisms underlying IgE-associated allergic disorders.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Precision medicine allergy immunoassay methods for assessing immunoglobulin E sensitization to aeroallergen molecules
Popescu FD, et al.
World journal of methodology, 8, 17-17 (2018)
Factors affecting human IgE and IgG responses to allergen-like Schistosoma mansoni antigens: Molecular structure and patterns of in vivo exposure
Fitzsimmons CM, et al.
International Archives of Allergy and Immunology, 142, 40-50 (2019)
Allergic and nonallergic forms of atopic diseases
Novak N and Bieber T
The Journal of Allergy and Clinical Immunology, 112(2), 252-262 (2003)
Immunoglobulin E in health and disease
Amarasekera M, et al.
Asia Pacific Allergy, 1(1), 12-15 (2011)
D G Ebo et al.
Cytometry. Part B, Clinical cytometry, 72(3), 196-203 (2006-11-18)
Correct identification of the culprit venom is a prerequisite for specific venom immunotherapy (VIT). Despite the efficacy of VIT, issues as how to monitor treatment and when to discontinue maintenance therapy remain to be established. To evaluate diagnostic performances of
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