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生物来源
mouse
质量水平
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
CXN-6, monoclonal
形式
buffered aqueous solution
种属反应性
chicken, rat, feline, bovine, human, porcine, mouse
包装
antibody small pack of 25 μL
浓度
~1.0 mg/mL
技术
immunoblotting: 1:5,000-1:7,500 using mouse myoblast C2C12 cell line extract
immunofluorescence: 1:500-1:1,000 using mouse myoblast C2C12 cell line
immunohistochemistry: 1:200-1:500 using heat-retrieved formalin-fixed, paraffin-embedded mouse heart sections
同位素/亚型
IgM
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
bovine ... Gja1(281193)
cat ... Gja1(101100211)
chicken ... Gja1(395278)
human ... GJA1(2697)
mouse ... Gja1(14609)
rat ... Gja1(24392)
一般描述
Connexin 43 (Cx43) is also known as gap junction alpha-1 protein (GJA1). It is mapped to human chromosome 6q22.31. Cx43 is membrane protein with four transmembrane spanning regions, which comprise the gap junction channel. The loop connecting the transmembrane regions play a key role in channel docking. The N- and C-terminal amino acids are exposed to cytoplasm.
免疫原
synthetic peptide from the C-terminal region of Connexin 43 protein, conjugated to KLH
生化/生理作用
Connexin 43 (Cx43) function is controlled by phosphorylation, SUMOylation and ubiquitination. The C-terminal tail of Cx43 modulates cytoskeletal dynamics and cell migration properties. Mutation in Cx43 leads to dysfunction of channel regulation domain and is associated with pathogenesis of hypoplastic left heart syndrome (HLHS). Cx43 dysregulation in testis contributes to infertility and testicular tumor. A gene mutation in the Cx43 results in abnormal development of eyes and teeth in oculodentodigital dysplasia (ODDD) , craniometaphyseal dysplasia and sudden infant death syndrome. Mutations in the GJA1 results in abnormal facial and skull development in hallermann-streiff syndrome (HSS). Missense mutations in GJA1 gene is also implicated in skin disorder called the, erythrokeratodermia variabilis et progressiva (EKVP)
外形
The product is supplied as a culture supernatant solution containing 15 mM sodium azide as a preservative. The product contains bovine serum albumin and a human-derived protein.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品--人源产品
Cell & bioscience, 12(1), 191-191 (2022-12-03)
Mechanical loading promotes bone formation and osteocytes are a major mechanosensory cell in the bone. Both Piezo1 channels and connexin 43 hemichannels (Cx43 HCs) in osteocytes are important players in mechanotransduction and anabolic function by mechanical loading. However, the mechanism
Structural organization of gap junction channels
Biochimica et Biophysica Acta - Biomembranes, 1711(2), 99-125 (2005)
Identification of connexin43 (alpha1) gap junction gene mutations in patients with hypoplastic left heart syndrome by denaturing gradient gel electrophoresis (DGGE)
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 479(1), 173-186 (2001)
Dominant de novo mutations in GJA1 cause erythrokeratodermia variabilis et progressiva, without features of oculodentodigital dysplasia
The Journal of Investigative Dermatology, 135(6), 1540-1547 (2015)
The gap junction channel protein connexin 43 is covalently modified and regulated by SUMOylation
The Journal of Biological Chemistry, 287(19), 15851-15861 (2012)
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