生物来源
mouse
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
SC-35, monoclonal
形式
buffered aqueous solution
分子量
~35 kDa
种属反应性
human, mouse, quail, hamster, frog, porcine, rat
浓度
~1.0 mg/mL
技术
ELISA: suitable
dot blot: suitable
immunoblotting: suitable
immunofluorescence: 0.125-0.25 μg/mL using HeLa cells
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... SRSF2(6427)
一般描述
小鼠抗-剪接因子SC-35单克隆抗体（小鼠IgG1同种型）来源于小鼠骨髓瘤细胞与部分纯化剪接体免疫的RBF-DHJ小鼠脾细胞融合产生的SC-35杂交瘤。剪接因子SC-35，也称为丝氨酸精氨酸二肽富含性剪切因子2(SRSF2)，属于丝氨酸精氨酸二肽富含性(SR)蛋白家族。
免疫原
HeLa细胞制备的部分纯化剪接体
应用
抗-剪接因子SC-35抗体已用于
- 斑点印迹
- 酶联免疫吸附测定(ELISA)
- 免疫荧光
- 免疫组织化学
- 免疫印迹
- 免疫沉淀
生化/生理作用
剪接因子SC35是pre-mRNA组成性和选择性剪接的重要调控因子。它控制基因毒性应激下的细胞凋亡。丝氨酸/精氨酸蛋白激酶1(SRPK1)可特异性磷酸化SC-35，导致p53抑制和周期蛋白D1上调。SC-35浓度升高与肺鳞状细胞癌和肺腺癌等多种癌症的侵袭性表型有关。SC35在人乳头瘤病毒(HPV)感染细胞的肿瘤进展中起致癌作用。
外形
溶于0.01 M磷酸盐缓冲液（pH 7.4）中，含有15 mM叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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Proceedings of the National Academy of Sciences of the United States of America, 119(25), e2206046119-e2206046119 (2022-06-16)
Nuclear speckles are non-membrane-bound organelles known as storage sites for messenger RNA (mRNA) processing and splicing factors. More recently, nuclear speckles have also been implicated in splicing and export of a subset of mRNAs, including the influenza virus M mRNA
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