跳转至内容
Merck
CN
所有图片(3)

文件

安全信息

SAB4200716

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

enhanced validation

clone VIM-13.2, purified from hybridoma cell culture

别名:

Anti-F5H288

登录查看公司和协议定价
所有图片(3)

About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

200

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

VIM-13.2, monoclonal

形式

buffered aqueous solution

分子量

~58 kDa

种属反应性

mouse, bovine, canine, rat, monkey, human, chicken

增强验证

independent
Learn more about Antibody Enhanced Validation

浓度

~1.0 mg/mL

技术

immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast HS68 cell line extract
immunofluorescence: 1.25-2.5 μg/mL using human foreskin fibroblast HS68 cells
immunohistochemistry: suitable

同位素/亚型

IgM

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

bovine ... Vim(280955)
chicken ... Vim(420519)
dog ... Vim(477991)
human ... VIM(7431)
mouse ... Vim(22352)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

相关类别

一般描述

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the VIM-13.2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with human foreskin fibroblast extract. Vimentin is a type III intermediate filaments (IF) expressed in a wide range of cell types. including, pancreatic precursor cells, fibroblasts and sertoli cells. It is also expressed in trophoblastic giant cells, neuronal precursor cells, lung epithelial cells, renal tubular and stromal cells. Vimentin is also present in leucocytes, macrophages, neutrophils and mesangial cells.

免疫原

human foreskin fibroblast extract

应用

Anti-Vimentin antibody, Mouse monoclonal has been used in:
  • western blotting
  • indirect immunofluorescence
  • immunohistochemistry

生化/生理作用

Vimentin plays a significant role in supporting and anchoring the cellular position of organelles, such as nucleus, endoplasmic reticulum, mitochondria (either laterally or terminally). It helps to maintain the mesenchymal cells especially their shape, motility, survival during of mechanical stress. Epithelial cancers like prostate, breast, lung show an increased vimentin expression. Vimentin is also highly expressed in gastrointestinal tumors, central nervous system tumors and malignant melanoma.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our 产品选型工具.

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

Podoplanin regulates mammary stem cell function and tumorigenesis by potentiating Wntbeta-catenin signaling
Bresson L, et al.
Development, 145(4), dev160382-dev160382 (2018)
Morphological and quantitative analysis on alpha-tubulin modifications in glioblastoma cells
Gadau SD
Neuroscience Letters, 687, 111-118 (2018)
The type III intermediate filament vimentin regulates organelle distribution and modulates autophagy
Biskou O, et al.
Testing, 14(1), e0209665-e0209665 (2019)
Laura Bresson et al.
Development (Cambridge, England), 145(4) (2018-01-24)
Stem cells (SCs) drive mammary development, giving rise postnatally to an epithelial bilayer composed of luminal and basal myoepithelial cells. Dysregulation of SCs is thought to be at the origin of certain breast cancers; however, the molecular identity of SCs
Taito Miyake et al.
Scientific reports, 10(1), 14292-14292 (2020-09-02)
Peritoneal fibrosis (PF) is a severe complication of peritoneal dialysis, but there are few effective therapies for it. Recent studies have revealed a new biological function of trehalose as an autophagy inducer. Thus far, there are few reports regarding the
查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门