产品名称
抗VSV 糖蛋白抗体，小鼠单克隆, clone P5D4, purified from hybridoma cell culture
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P5D4, monoclonal
form
buffered aqueous solution
species reactivity
virus (Vesicular stomatitis virus (VSV))
packaging
antibody small pack of 25 μL
concentration
~1 mg/mL
technique(s)
immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunocytochemistry: suitable
immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein.
immunoprecipitation (IP): suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Application
小鼠抗VSV糖蛋白单克隆抗体可用于免疫印迹、免疫荧光、免疫沉淀、免疫细胞化学、透射电镜（TEM）和应用显微注射抗体的研究中。
Biochem/physiol Actions
水泡性口腔炎病毒糖蛋白（VSV-G）可构建一个具有吸引力的模型，从而用于研究膜蛋白的成熟和细胞内运输。它可介导VSV附着在细胞表面，并诱导病毒和靶膜之间的pH依赖性融合。此外，它还具有在其胞质域内进行细胞内分选的信息，包括从内质网（ER）高效输出、基底外侧传递和胞吞作用。高尔基体池之间的单向运输已使用含有VSV-G的高尔基体膜进行了证明。VSV-G的温度敏感突变体可用于研究内质网折叠中间体的存在。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
小鼠单克隆抗VSV糖蛋白抗体（小鼠IgG1同种型）衍生自杂交瘤P5D4，该杂交瘤是通过小鼠骨髓瘤细胞与经水泡性口腔炎病毒糖蛋白（VSV-G）的合成肽（与KLH缀合）免疫的BALB/c小鼠脾细胞融合而产生的。
Physical form
溶于含有 15 mM 叠氮化钠的 0.01M 磷酸盐缓冲盐水（pH 7.4）溶液中。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
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常规特殊物品
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