推荐产品
生物来源
rat
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
2F11H5, monoclonal
表单
buffered aqueous solution
种属反应性
human
浓度
~1 mg/mL
技术
flow cytometry: 5-10 μg/test using using HeLa cells
immunofluorescence: 20 μg/mL using using human HeLa cells.
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... CHD1(1105)
相关类别
一般描述
Monoclonal Anti-CHD1 (rat IgG2a isotype) is derived from the hybridoma 2F11H5 produced by the fusion of mouse myeloma cells and lymph node cells from rat immunized with a synthetic peptide. Chromodomain-helicase-DNA-binding protein 1 (CHD1) is an ATP-dependent chromatin-remodeling factor expressed majorly in cerebellum and basal ganglia. CHD1 gene is mapped to human chromosome 5q15-q21.1. CHD1 comprises two chromodomains.
特异性
Monoclonal Anti-CHD1 recognizes human CHD1.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal region of human CHD1.
应用
Monoclonal Anti-CHD1 antibody produced in rat may be used in:
- immunofluorescence
- flow cytometry
- immunoblotting
- immunohistochemistry
生化/生理作用
Chromodomain-helicase-DNA-binding protein 1 (CHD1) interacts with the trimethylated lysine residues of histones and alters access of the transcriptional apparatus to its chromosomal DNA template. CHD1 is required for maintenance of open chromatin and pluripotency in embryonic stem cells. Missense mutation in the CHD1 gene is implicated in the Pilarowski-Bjornsson syndrome as well as in speech delay and facial dismorphism.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2–8 °C for up to one month. For extended storage,freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Missense variants in the chromatin remodeler CHD1 are associated with neurodevelopmental disability
Pilarowski GO, et al.
Journal of medical Genetics, 55(8), 561-566 (2018)
Familial segregation of a 5q15-q21. 2 deletion associated with facial dysmorphism and speech delay
Zepeda-Mendoza
Clinical case reports, 7(6), 1154-1160 (2019)
Lena Ho et al.
Nature, 463(7280), 474-484 (2010-01-30)
New methods for the genome-wide analysis of chromatin are providing insight into its roles in development and their underlying mechanisms. Current studies indicate that chromatin is dynamic, with its structure and its histone modifications undergoing global changes during transitions in
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