SAB4200625
Anti-Methyl-Histone H3 (Me-Lys9)(H3K9me1) antibody, Mouse monoclonal
clone 7E7-H12, purified from hybridoma cell culture
别名:
9430068D06RIK, H3.3A, H3.3B, H3F3, H3F3A, H3F3A/H3F3B, H3F3B, HISTONE 3B, LOC100045490, RP11−396C23.1, wu:fb58e10, zgc:56193, zgc:86731
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About This Item
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
7E7-H12, monoclonal
形式
buffered aqueous solution
分子量
antigen ~17 kDa
种属反应性
human
浓度
~1 mg/mL
技术
immunoblotting: 2.5-5 μg/mL using human HeLa cells.
immunocytochemistry: 2.5-5 μg/mL using human HeLa cells.
同位素/亚型
IgG1
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
monomethylation (Lys9)
基因信息
human ... H3C1(8350)
一般描述
Anti-Methyl-Histone H3 (Me-Lys9) (H3K9me1) antibody, Mouse Monoclonal (mouse IgG1 isotype) is derived from the hybridoma 7E7-H12 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a methylated (Me-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH.
免疫原
Methylated (Me-Lys9) peptide corresponding to the N-terminus of human histone H3, conjugated to KLH.
应用
Anti-Methyl-Histone H3 (Me-Lys9) (H3K9me1) antibody has been used in immunoblotting and immunocytochemistry.
生化/生理作用
Histones are subjected to extensive covalent modifications that play an important role in development and in cancer. These modifications include phosphorylation, methylation, acetylation and ubiquitination. Histones H3 and H4 are the predominant histones modified by methylation and are highly methylated in mammalian cells. Histone methylation, like acetylation, is a complex, dynamic process involving several processes, including transcriptional regulation, chromatin condensation, mitosis, and heterochromatin assembly. Moreover, lysine residues can be mono-, di-, and tri-methylated, adding further complexity to the regulation of chromatin structure. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. Methylation of H3 at Lys9 is a modification intrinsically linked to epigenetic silencing and heterochromatin assembly.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Methylation of histone H3 at lysine 4 is highly conserved and correlates with transcriptionally active nuclei in Tetrahymena
Proceedings of the National Academy of Sciences of the USA, 96(26), 14967-14972 (1999)
Chromatin modifications and their function
Cell, 128(4), 693-705 (2007)
Cancer epigenetics: from mechanism to therapy
Cell, 150(1), 12-27 (2012)
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