生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~32 kDa
种属反应性
mouse, human, rat
浓度
~1.0 mg/mL
技术
immunoblotting: 1-2 μg/mL using lysates of rat brain (S1 fraction)
immunoprecipitation (IP): 5-10 μg using lysates of mouse brain (S1 fraction).
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAPRE3(22924)
一般描述
End-binding protein-3 (EB3), also known as microtubule-associated protein RP/EB 3 (MAPRE3), belongs to a distinct family of “plus-end tracking proteins” (+TIPs). EB proteins are among the most conserved components of the microtubules cytoskeleton. EB proteins contain an N-terminal calponin homology (CH) domain and a unique EB-like C-terminal motif. EB3 is exclusively expressed in the central nervous system and it interacts with a CNS-specific APC homolog APCL.
免疫原
Synthetic peptide corresponding to an internal sequence of human EB3, conjugated to KLH. The corresponding sequence is highly conserved (single amino acid substitution) in rat and mouse EB3.
应用
Anti-EB3 antibody produced in rabbit has been used in:
- immunoblotting
- co-immunoprecipitation
- immunocytochemistry
- glutathione S-transferase (GST) pull-down assay
生化/生理作用
In neurons, End-binding protein-3 (EB3) has been shown to interact with scaffold protein ankyrin-G and PSD95 and is required for stabilizing neuronal polarity in mature neurons and in determining neuronal dendritic morphology. EB3 is also involved in MT polymerization and cell migration. EB3 may be involved in stabilizing MTs and anchoring them to centrosomes during spindle formation. Knockout of EB3 inhibits myoblasts elongation and fusion into myotubes.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Plus-end-tracking proteins and their interactions at microtubule ends
Current Biology, 20(12), R528-R537 (2010)
Stim2-Eb3 association and morphology of dendritic spines in hippocampal neurons
Scientific reports, 7(1), 17625-17625 (2017)
EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue
Oncogene, 19(2), 210-210 (2000)
PSD-95 alters microtubule dynamics via an association with EB3
The Journal of Neuroscience, 31(3), 1038-1047 (2011)
End-binding proteins EB3 and EB1 link microtubules to ankyrin G in the axon initial segment
Proceedings of the National Academy of Sciences of the USA, 108(21), 8826-8831 (2011)
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