SAB4200598
Monoclonal Anti-FTH1 antibody produced in mouse
~1.0 mg/mL, clone FTH24, purified immunoglobulin
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Monoclonal Anti-FHC, Monoclonal Anti-FTH, Monoclonal Anti-FTH1, Monoclonal Anti-FTHL6, Monoclonal Anti-OK/SW-cl.84, Monoclonal Anti-PIG15, Monoclonal Anti-PLIF, Monoclonal Anti-cell proliferation-inducing gene 15 protein, Monoclonal Anti-ferritin H subunit, Monoclonal Anti-ferritin heavy chain, Monoclonal Anti-ferritin, heavy polypeptide 1, Monoclonal Anti-placenta immunoregulatory factor, Monoclonal Anti-proliferation-inducing protein 15
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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
FTH24, monoclonal
形式
buffered aqueous solution
种属反应性
human
浓度
~1.0 mg/mL
技术
flow cytometry: 10-20 μg/test using HeLa cells
immunoblotting: 0.5-1.0 μg/mL using total cell extracts of HEK-293T cells overexpressing FTH1 (expected: ~26kDa).
indirect immunofluorescence: 5-10 μg/mL using HeLa cells
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... FTH1(2495)
相关类别
一般描述
Monoclonal Anti-FTH1 (mouse IgG1 isotype) is derived from the hybridoma FTH24 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal sequence of human FTH1. Ferritin, a highly conserved iron-binding protein, is composed of two subunits, namely a 21 kDa heavy (H) chain and a 19 kDa light (L) chain. Ferritin heavy chain 1 gene is mapped on human chromosome 11q13. It is mainly found in heart and pancreas.
特异性
Monoclonal Anti- FTH1 recognizes human FTH1.
免疫原
synthetic peptide corresponding to an internal sequence of human FTH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
应用
Monoclonal Anti-FTH1 antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- flow cytometry
- immunofluorescence
生化/生理作用
Ferritin heavy chain catalyzes the first step in iron storage, the oxidation of Fe(II) to Fe(III). In addition to iron buffering, heavy chain ferritin also enhances de novo thymidine biosynthesis. Serum ferritin level is the most commonly used marker for determining iron status stored in the body. It is markedly influenced by factors such as inflammation, malnutrition, malignancy, and iron overload disorders. Studies have found that defects in ferritin proteins are also associated with several neurodegenerative diseases. FTH1 can also be used as a possible prognostic marker for breast cancer.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Heavy chain ferritin enhances serine hydroxymethyltransferase expression and de novo thymidine biosynthesis
The Journal of Biological Chemistry, 276(23), 19855-19861 (2001)
Effects of pulsed electromagnetic fields on human osteoblastlike cells (MG-63): a pilot study
Ferroelectrics, 468(8), 2260-2277 (2019)
Ferritin heavy chain in triple negative breast cancer: a favorable prognostic marker that relates to a cluster of differentiation 8 positive (CD8+) effector T-cell response
Molecular and Cellular Proteomics, 13(7), 1814-1827 (2014)
Could a dysfunction of ferritin be a determinant factor in the aetiology of some neurodegenerative diseases?
Biochim. Biophys. Acta Gen. Subj., 1800(8), 770-782 (2010)
Limitations of serum ferritin in diagnosing iron deficiency in inflammatory conditions
International journal of chronic diseases, 2018(23), 19855-19861 (2018)
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