生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
FMR2.5, monoclonal
形式
buffered aqueous solution
分子量
~71 kDa
种属反应性
rat, human, mouse, hamster
浓度
~1 mg/mL
技术
flow cytometry: 2.0-5.0 μg/test using HeLa cells
immunoblotting: 1.0-2.0 μg/mL using HepG2 total cell extracts.
immunocytochemistry: suitable
immunofluorescence: 5-10 μg/mL using HepG2 cells.
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... FMR1(2332)
mouse ... Fmr1(14265)
rat ... Fmr1(24948)
相关类别
一般描述
Monoclonal Anti-FMR1 (mouse IgG1 isotype) is derived from the hybridoma FMR2.5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Fragile X mental retardation 1 (FMR1) is encoded by the gene mapped to human chromosome Xq27.3q28. FMR1 localizes to both the nucleus and the cytoplasm.
特异性
Monoclonal Anti- FMR1 recognizes human, hamster, rat and mouse FMR1.
免疫原
synthetic peptide corresponding to a sequence at the C-terminal region of human FMR1
应用
Monoclonal Anti-FMR1 antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- immunofluorescence
- flow cytometry
生化/生理作用
Fragile X mental retardation 1 (FMR1) protein functions as an RNA-binding protein that associates with polyribosomes and is a likely component of a messenger ribonuclear protein (mRNP) particle. Since, FMR1 contains both a nuclear localization signal and a nuclear export signal it is also implicated in nucleocytoplasmic transport. Mutation in the gene leads to the development of fragile X mental retardation (FXMR) syndrome.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For extended storage, freeze at 20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Familial interstitial Xq27.3q28 duplication encompassing the FMR1 gene but not the MECP2 gene causes a new syndromic mental retardation condition
Rio M,et al.
European Journal of Human Genetics, 18, 285-285 (2010)
FMRP Associates with Polyribosomes as an mRNP, and the I304N Mutation of Severe Fragile X Syndrome Abolishes This Association
Feng Y, et al.
Molecular Cell, 1, 109-118 (1997)
Fragile X Mental Retardation Protein FMRP Binds mRNAs in the Nucleus
Kim M, et al.
Molecular and Cellular Biology, 29, 214-228 (2009)
Kan Yang et al.
Cell reports, 37(5), 109939-109939 (2021-11-04)
Autism spectrum disorder (ASD) is a highly heritable neurodevelopmental disorder, causing defects of social interaction and repetitive behaviors. Here, we identify a de novo heterozygous gene-truncating mutation of the Sentrin-specific peptidase1 (SENP1) gene in people with ASD without neurodevelopmental delay.
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