SAB4200572
Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal
clone NSE-P2, purified from hybridoma cell culture
别名:
Monoclonal Anti-2-phospho-D-glycerate hydro-lyase, Monoclonal Anti-2-phospho-D-glycerate hydrolyase, Monoclonal Anti-Enolase 2 (gamma, neuronal), Monoclonal Anti-NSE, Monoclonal Anti-Neural enolase, Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse, Monoclonal Anti-gamma-enolase
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关于此项目
NACRES:
NA.41
UNSPSC Code:
12352203
Species reactivity:
human, rat
Conjugate:
unconjugated
Application:
immunohistochemistry
western blot
western blot
Clone:
NSE-P2, monoclonal
Citations:
6
Technique(s):
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
Uniprot accession no.:
产品名称
Anti-Neuron-Specific Enolase (NSE) antibody, Mouse monoclonal, clone NSE-P2, purified from hybridoma cell culture
species reactivity
human, rat
concentration
~1 mg/mL
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
NSE-P2, monoclonal
form
buffered aqueous solution
mol wt
antigen ~47 kDa
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum.
western blot: 2.5-5.0 μg/mL using SH-SY5Y total cell extracts.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ENO2(2026)
Application
Monoclonal Anti-Neuron-Specific Enolase (NSE) antibody produced in mouse may be used in:
- immunoblotting
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemistry
Biochem/physiol Actions
Monoclonal Anti-Neuron-specific Enolase (NSE) recognizes human and rat NSE.
Neuron-specific enolase (NSE) catalyzes the production of phosphoenolpyruvate from 2-phospho-D-glycerate in the glycolytic pathway. NSE is a specific neurobiochemical marker of brain damage after traumatic brain injury, hypoxic encephalopathy and stroke. Moreover, NSE has been suggested to be a general circulating marker for neuroendocrine tumors. Interestingly, high levels of NSE were also reported in breast cancer upon exposure to the environmental pollutants arsenite and cadmium.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-Neuron-specific Enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Neuron-specific enolase (NSE) is present in the brain especially in the neuroendocrine cells and neurons. It is a cytosolic enzyme, that exists as a dimer and belongs to the family of enolase enzymes. NSE gene is mapped to human chromosome12p13.31.
Immunogen
synthetic peptide corresponding to an internal sequence of human NSE.1 The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
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DalBMG, et al.
Journal of Translational Medicine, 17(1), 74-74 (2019)
Circulating biomarkers in gastroenteropancreatic neuroendocrine tumours
Oberg K
Endocrine-Related Cancer, 18(S1), S17-S25 (2011)
New insights into the role of neuron-specific enolase in tic disorders
Hao J, et al.
Neurological Sciences, 40(6), 1167-1172 (2019)
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