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安全信息

SAB4200547

Sigma-Aldrich

Anti-IDH1 (R132S) antibody, Mouse monoclonal

clone SMab-1, purified from hybridoma cell culture

别名:

Monoclonal Anti-IDCD, Monoclonal Anti-IDH, Monoclonal Anti-IDH1 (R132S) antibody produced in mouse, Monoclonal Anti-IDP, Monoclonal Anti-IDPC, Monoclonal Anti-NADP(+)-specific ICDH, Monoclonal Anti-NADP-dependent isocitrate dehydrogenase, cytosolic, Monoclonal Anti-Oxalosuccinate decarboxylase, Monoclonal Anti-PICD, Monoclonal Anti-isocitrate dehydrogenase 1 (NADP+), soluble, NADP-dependent isocitrate dehydrogenase, peroxisomal

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

SMab-1, monoclonal

形式

buffered aqueous solution

分子量

antigen ~43 kDa

种属反应性

human

浓度

~1 mg/mL

技术

immunoblotting: 0.25-0.5 μg/mL using using total cell extracts of HEK-293T cells overexpressing IDH1R132S

UniProt登记号

O75874

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

mutation (Arg132Ser)

基因信息

human ... IDH1(3417)

一般描述

Monoclonal Anti-IDH1 (R132S) (mouse IgG1 isotype) is derived from the hybridoma SMab1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized by a peptide. Isocitrate dehydrogenase 1 (IDH1) is a homodimeric, nicotinamide adenine dinucleotide phosphate (NADP)-specific enzyme. It is localized in the peroxisomes and cytoplasm. IDH1 is mapped on the human chromosome at 2q34.

特异性

Monoclonal Anti- IDH1 (R132S) recognizes only human R132S mutated IDH1 and does not cross react with others.

免疫原

immunized a peptide corresponding to the mutation R132S of human IDH1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

应用

Monoclonal Anti-IDH1 (R132S) antibody produced in mouse may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

生化/生理作用

Isocitrate dehydrogenase (IDH) plays an important role in catalyzing the oxidative decarboxylation of isocitrate to α-ketoglutarate (αKG). Mutations in this gene are associated with several cancers, such as glioblastoma, acute myeloid leukemia (AML) and chondrosarcoma. Mutation in IDH1 specific to Arg132 results in the formation of 2-hydroxyglutarate (2HG) instead of αKG. 2HG hinders DNA and histone methylation resulting in alteration of gene transcription.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Screen for IDH1, IDH2, IDH3, D2HGDH and L2HGDH mutations in glioblastoma
Krell D, et al.
PLoS ONE, 6(5), e19868-e19868 (2011)
Molecular pathogenesis of IDH mutations in gliomas
Ichimura Koichi
Brain Tumor Pathology, 29(3), 131-139 (2012)
IDH mutation impairs histone demethylation and results in a block to cell differentiation
Lu C, et al.
Nature, 483(7390), 474-478 (2012)
Altered cancer cell metabolism in gliomas with mutant IDH1 or IDH2
Borodovsky A, et al.
Current Opinion in Oncology, 24(1), 83-83 (2012)

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