SAB4200543
Anti-Calbindin-D-28K antibody, Mouse monoclonal
clone CB-955, purified from hybridoma cell culture
别名:
Monoclonal Anti-(28kD), Monoclonal Anti-D-28K, Monoclonal Anti-D-dependent calcium-binding protein, Monoclonal Anti-RTVL-H protein, Monoclonal Anti-avian-type, Monoclonal Anti-calbindin 1, Monoclonal Anti-vitamin
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关于此项目
NACRES:
NA.44
UNSPSC Code:
12352203
Conjugate:
unconjugated
Clone:
CB-955, monoclonal
Application:
immunohistochemistry
western blot
western blot
Species reactivity:
mouse, human, rat
Citations:
17
Technique(s):
immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.
western blot: 2-4 μg/mL using MDBK extract.
western blot: 2-4 μg/mL using MDBK extract.
产品名称
Anti-Calbindin-D-28K antibody, Mouse monoclonal, clone CB-955, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
CB-955, monoclonal
form
buffered aqueous solution
mol wt
antigen ~28 kDa
species reactivity
mouse, human, rat
enhanced validation
independent
Learn more about Antibody Enhanced Validation
concentration
~1.0 mg/mL
technique(s)
immunohistochemistry: 10-20 μg/mL using formalin-fixed, paraffin embedded rat cerebellum.
western blot: 2-4 μg/mL using MDBK extract.
isotype
IgG1
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... CALB1(793)
Application
- Anti-Calbindin-D-28K antibody, Mouse monoclonal has been used in:
- western blotting
- enzyme-linked immunosorbent assay (ELISA){31
- immunohistochemistry
- immunohistochemical procedure
- indirect immunofluorescence
Biochem/physiol Actions
Calbindin D-28k might play a vital role as an intracellular Ca2+ buffer. It has an ability to protect nerve cells from calcium-mediated neurotoxicity. The encoded protein functions as a potent neuroanatomical marker which can be used to selectively visualize certain neurons and pathways in the central nervous system and peripheral nervous system. Mutations in the gene has been observed in patients with Parkinson′s disease (PD).
Calbindin-D (28K) functions as a mobile or partly immobilized Ca2+ -buffer with medium kinetics and affinity buffer function. Second, it functions in buffered Ca2+- diffusion-transport function. Finally, it interacts with target proteins in its apo-and Ca2+-loaded form-sensor- like function. Over-expression of this protein stimulates neurite outgrowth in dopaminergic neuronal cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Anti-Calbindin-D-28K antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CB-955 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a purified bovine kidney calbindin-D-28K.
Calbindin-D-28K (CALB1) is encoded by the gene mapped to human chromosome 8q21.3-q22.1. CALB1 belongs to the EF hand superfamily of Ca2+ binding proteins (CBPs). The encoded protein contains EF hand motif involved in the specific and reversible binding of Ca2+. It is highly expressed in specific regions of central nervous system such as cerebellar Purkinje cells and hippocampal granule cells.
Immunogen
purified bovine kidney calbindin-D-28K. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2). The antibody is purified from culture supernatant of hybridoma cells grown in a bioreactor.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Calbindin-D28K-containing neurons in animal models of neurodegeneration: possible protection from excitotoxicity
Iacopino A
Brain Research Molecular Brain Research, 13, 251-261 (1992)
Overexpression of calbindin-D28K induces neurite outgrowth in dopaminergic neuronal cells via activation of p38 MAPK
Choi WS, et al.
Biochemical and biophysical research communications, 287(3), 656-661 (2001)
Central role of the proximal tubular alpha Klotho/FGF receptor complex in FGF23-regulated phosphate and vitamin D metabolism
Takeshita A, et al.
Scientific reports, 8(1), 6917-6917 (2018)
Calbindin D-28k and parvalbumin in the rat nervous system
Celio MR
Neuroscience, 35, 375-475 (1990)
A splice site variant in INPP5E causes diffuse cystic renal dysplasia and hepatic fibrosis in dogs
Dillard KJ, et al.
Testing, 13(9) (2018)
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