所有图片(2)
别名:
Anti-136 kDa double-stranded RNA-binding protein, Anti-DRADA, Anti-DSH, Anti-G1P1, Anti-IFI-4, Anti-IFI4, Anti-K88DSRBP, Anti-P136, Anti-adenosine deaminase acting on RNA 1-A, Anti-adenosine deaminase, RNA-specific, Anti-double-stranded RNA-specific adenosine deaminase, Anti-interferon-induced protein 4
UNSPSC代码:
12352203
NACRES:
NA.41
推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~110 kDa
种属反应性
human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 10-20 μg using lysates of human HepG2 or HEK-293T cells.
indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells.
western blot: 2-4 μg/mL using whole extracts of human HEK-293T cells.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
bovine ... ADAR(505134)
human ... ADAR(103)
mouse ... Adar(56417)
rat ... Adar(81635)
一般描述
Adenosine deaminase that acts in RNA (ADAR) exists in two isoforms namely, ADAR1 and ADAR2, in mammals. Both the isoforms are expressed in variety of tissues but in general, the pre-mRNA being edited represents receptors of the central nervous systems. ADAR1 has several isoforms which include the full length ADAR1 (150 kDa) and two functionally active short isoforms (80 and 110 kDa).
免疫原
synthetic peptide corresponding to an internal s of human ADAR1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, bovine, pig and monkey.
应用
Anti-ADAR1 antibody produced in rabbit has been used in
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
Adenosine deaminase that acts in RNA 1 (ADAR1) play an important role in A-to-I RNA editing. In addition, it is also implicated in differentiating cells of embryo and adult tissues, facilitating cell survival and permitting their further differentiation and maturation. Its role in embryonic development is specifically observed within the hematopoietic lineage as well as in adult hematopoietic progenitor cells (HPCs). ADAR1 plays a key role in hematopoietic stem cell maintenance. It also block interferon signaling, that might help organisms to fight against the harmful effects of interferon activation linked with various diseases, such as chronic inflammation, autoimmune disorders and cancer.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Guermarie Velazquez-Torres et al.
Nature communications, 9(1), 461-461 (2018-02-02)
Previously we have reported that metastatic melanoma cell lines and tumor specimens have reduced expression of ADAR1 and consequently are impaired in their ability to perform A-to-I microRNA (miRNA) editing. The effects of A-to-I miRNAs editing on melanoma growth and
A-to-I miR-378a-3p editing can prevent melanoma progression via regulation of PARVA expression
Velazquez-Torres G, et al.
Nature Communications, 9(1), 461-461 (2018)
ADAR1 is required for hematopoietic progenitor cell survival via RNA editing
XuFeng R, et al.
Proceedings of the National Academy of Sciences of the USA, 106(42), 17763-17768 (2009)
ADAR1 is essential for the maintenance of hematopoiesis and suppression of interferon signaling
Hartner JC, et al.
Nature Immunology, 10(1), 109-109 (2009)
RNA editing catalyzed by ADAR1 and its function in mammalian cells
Wang Q
Biochemistry (Moscow), 76(8), 900-900 (2011)
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