推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~45 kDa
种属反应性
mouse, human, rat
浓度
~0.1 mg/mL
技术
immunoprecipitation (IP): 40 μL using HEK-293T cells overexpressing human VASP
western blot: 1:5000-10000 using human platelets cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pThr278)
基因信息
human ... VASP(7408)
mouse ... Vasp(22323)
rat ... Vasp(361517)
一般描述
VASP is localized at highly dynamic membrane regions, focal adhesion sites, lamellipodia protrusions, filopodia tips, and along stress fibers. It is also localized at cell-matrix and cell-cell contacts.
Vasodilator-stimulated phosphoprotein (VASP) is located on human chromosome 19q13.32. It belongs to enabled (Ena) family of proteins and encodes a protein of 46 kDa. VASP has three domain organisation, that is, an N-terminal Ena/VASP (EVH1) homology domain, proline rich central domain and EVH2 actin binding domain.
特异性
Anti-phospho-VASP [pThr278] specifically recognizes human VASP phosphorylated at Thr278.
免疫原
synthetic peptide containing phosphorylated Thr278 of human VASP, conjugated to KLH. The corresponding sequence is identical in mouse and rat VASP.
应用
Anti-phospho-VASP [pThr278] antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
生化/生理作用
Regulation of phosphorylation of Vasodilator-stimulated phosphoprotein (VASP) is observed during actin cytoskeleton formation. It promotes protrusion and propulsion speed and inhibits branching of actins. It plays a key role in the signal transduction pathway related to neutrophil spreading.
VASP plays an important role in cell motility and adhesion. This protein is also involved in adherens junction formation and stabilization in epithelial cells. VASP is a substrate for cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP)-dependent protein kinases. VASP is required for endothelial barrier function in vivo. Knockout of VASP proteins in mice leads to increased endothelial permeability causing fatal vascular leakage and hemorrhage during late embryonic development.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Differential VASP phosphorylation controls remodeling of the actin cytoskeleton
Benz PM, et al.
Journal of Cell Science, 122(21), 3954-3965 (2009)
AMP-activated protein kinase impairs endothelial actin cytoskeleton assembly by phosphorylating vasodilator-stimulated phosphoprotein
Blume C, et al.
The Journal of Biological Chemistry, 282(7), 4601-4612 (2007)
M Reinhard et al.
The EMBO journal, 14(8), 1583-1589 (1995-04-18)
Profilins are small proteins that form complexes with G-actin and phosphoinositides and are therefore considered to link the microfilament system to signal transduction pathways. In addition, they bind to poly-L-proline, but the biological significance of this interaction is not yet
Cloning of the VASP (vasodilator-stimulated phosphoprotein) genes in human and mouse: structure, sequence, and chromosomal localization
Zimmer M, et al.
Genomics, 36(2), 227-233 (1996)
Relaxing the actin cytoskeleton for adhesion and movement with Ena/VASP
Trichet L, et al.
The Journal of Cell Biology, 181(1), 19-25 (2008)
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