SAB4200502
Anti-NONO antibody, Mouse monoclonal
clone NC5, purified from hybridoma cell culture
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Monoclonal Anti-52 kDa subunit, Monoclonal Anti-54 kDa nuclear RNA- and DNA-binding protein, Monoclonal Anti-55 kDa nuclear protein, Monoclonal Anti-DNA-binding p52/p100 complex, Monoclonal Anti-NMT55, Monoclonal Anti-NONO antibody produced in mouse, Monoclonal Anti-NRB54, Monoclonal Anti-P54, Monoclonal Anti-P54NRB, Monoclonal Anti-non-POU domain-containing octamer (ATGCAAAT) binding protein, Monoclonal Anti-p54(nrb)
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生物来源
mouse
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
NC5, monoclonal
形式
buffered aqueous solution
种属反应性
mouse, rat
浓度
~1.0 mg/mL
技术
indirect immunofluorescence: 1-2 μg/mL using whole extracts of mouse Hepa1-6 cells.
western blot: 0.5-1.0 μg/mL using whole extracts of mouse Hepa1-6 cells.
同位素/亚型
IgG2a
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Nono(53610)
rat ... Nono(317259)
一般描述
Monoclonal Anti-NONO (mouse IgG2a isotype) is derived from the hybridoma NC5 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Non-POU-domain-containing, octamer binding protein (NONO), also known as p54nrb, is a member of the DBHS (Drosophila behavior, human splicing) protein family. The family also consists of paraspeckle protein 1 (PSPC1) and splicing factor proline- and glutamine-rich (SFPQ). NONO, PSPC1 and SFPQ are the core protein components of paraspeckles.
特异性
Monoclonal Anti-NONO recognizes mouse NONO.
免疫原
Synthetic peptide corresponding to a sequence at the C-terminal region of mouse NONO, conjugated to KLH.1 The corresponding sequence is identical in rat and differs by a single amino acids in human.
应用
Monoclonal Anti-NONO antibody produced in mouse has been used in immunohistochemistry and immunoblotting.
生化/生理作用
Non-POU-domain-containing, octamer binding protein (NONO) complexes with androgen receptor and activates androgen receptor-mediated transcription. NONO is necessary for cAMP-dependent activation of cAMP response element-binding protein (CREB) target genes. It acts as a bridge between the CREB/ transducers of regulated CREB (TORC) complex and RNA polymerase II. NONO associates with Period circadian protein homolog 1 (PERIOD-1) protein and is essential for the maintenance of circadian rhythm. NONO along with the nuclear factor, polypyrimidine tract-binding protein-associated splicing factor (PSF) binds to defective RNA and are capable of their retention.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20°C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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