SAB4200479
Anti-SET antibody, Mouse monoclonal
clone SET51, purified from hybridoma cell culture
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Monoclonal Anti-2PP2A, Monoclonal Anti-I2PP2A, Monoclonal Anti-IGAAD, Monoclonal Anti-IPP2A2, Monoclonal Anti-PHAPII, Monoclonal Anti-SET antibody produced in mouse, Monoclonal Anti-SET nuclear oncogene, Monoclonal Anti-TAF-I, Monoclonal Anti-TAF-IBETA
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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
SET51, monoclonal
形式
buffered aqueous solution
分子量
antigen ~37 kDa
种属反应性
rat, human, mouse
浓度
~1.0 mg/mL
技术
western blot: 0.5-1.0 μg/mL using K562 total cell extracts.
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... SET(6418)
mouse ... Set(56086)
rat ... Set(307947)
一般描述
Monoclonal Anti-SET (mouse IgG2b isotype) is derived from the hybridoma SET51 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide. The SET (Suvar3-9, enhancer of zeste, trithorax) protein is localized to the nucleus and cytoplasm. It is characterized with a N-terminal domain with inhibitory activity and a highly acidic C-terminal domain that is involved in chromatin remodeling.
免疫原
peptide corresponding to a sequence within the internal region of human SET, conjugated to KLH. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).
应用
Monoclonal Anti-SET antibody produced in mouse has been used in immunoblotting and immunofluorescence.
生化/生理作用
Suvar3-9, enhancer of zeste, trithorax (SET) acts as a potential inhibitor of protein phosphatase 2A (PP2A) activity. It plays a key role in the regulation of normal and cancer signal transduction. In addition, it also regulates human natural killed-cell cytotoxicity by regulating natural killer cell IFN-γ production granzyme B gene expression through PP2A inactivation. SET has an ability to inhibit the tumor suppressor NM23-H1, a granzyme A DNAse-activated factor. It is also involved in negative regulation of histone acetylation. SET- nucleoporin 214 (Nup214)/CAN fusion protein might affect the normal regulation of PP2A and leads to the development of leukemogenesis.
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
SET/I2 PP 2A overexpression induces phenotypic, molecular, and metabolic alterations in an oral keratinocyte cell line
FEBS Journal, 284(17), 2774-2785 (2017)
Protein phosphatase 2A: a highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling
The Biochemical Journal, 353(3), 417-439 (2001)
The PP2A inhibitor SET regulates granzyme B expression in human natural killer cells
Blood, 117(8), 2378-2384 (2011)
The FEBS journal, 284(17), 2774-2785 (2017-06-22)
The multifunctional SET/I2PP2A protein is known to be overexpressed in head and neck squamous cell carcinoma. However, SET has been reported to have apparently conflicting roles in promoting cancer cell survival under oxidative stress conditions and preventing invasion and metastasis
Cell, 112(5), 659-672 (2003-03-12)
Granzyme A (GzmA) induces a caspase-independent cell death pathway characterized by single-stranded DNA nicks and other features of apoptosis. A GzmA-activated DNase (GAAD) is in an ER associated complex containing pp32 and the GzmA substrates SET, HMG-2, and Ape1. We
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