推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~49 kDa
种属反应性
mouse, rat, human
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... WIPI2(26100)
mouse ... Wipi2(74781)
rat ... Wipi2(288498)
一般描述
WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.
免疫原
synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.
应用
Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including
- immunoblotting
- immunoprecipitation
- immunostaining.
生化/生理作用
WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.
外形
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17
Matsui T, et al.
The Journal of cell biology, 217(8), 2633-2645 (2018)
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets
Tamura N, et al.
Febs Letters, 591(23), 3819-3830 (2017)
Kenta Imai et al.
Journal of cell science, 129(20), 3781-3791 (2016-09-03)
Autophagy is an intracellular degradation pathway conserved in eukaryotes. Among core autophagy-related (Atg) proteins, mammalian Atg9A is the sole multi-spanning transmembrane protein, and both of its N- and C-terminal domains are exposed to the cytoplasm. It is known that Atg9A
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Polson HE
Autophagy, 6(4), 506-522 (2010)
Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.
Proikas-Cezanne T
Journal of Cellular and Molecular Medicine, 15(9), 2007-2010 (2011)
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