SAB4200400
Anti-WIPI-2 (C-terminal) antibody produced in rabbit
~1.0 mg/mL, affinity isolated antibody
别名:
Anti-ATG18B, Anti-Atg21, Anti-CGI-50, Anti-WD repeat domain, phosphoinositide interacting 2, Anti-WIPI2
产品名称
Anti-WIPI-2 (C-terminal) antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~49 kDa
species reactivity
mouse, rat, human
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): 2.5-5.0 μg using lysates of rat NRK cells.
western blot: 2-4 μg/mL using whole extracts of human G-361 and NIH-3T3 cells.
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... WIPI2(26100)
mouse ... Wipi2(74781)
rat ... Wipi2(288498)
Application
Anti-WIPI-2 (C-terminal) antibody produced in rabbit may be used in various immunochemical techniques including
- immunoblotting
- immunoprecipitation
- immunostaining.
Biochem/physiol Actions
WIPI subfamily proteins acts as a key component of many essential biological functions such as signal transduction, transcription regulation and apoptosis. WIPI-2 is a phosphatidylinositol-3-phosphate binding protein that is needed for starvation induced autophagy. In addition, it is also essential for LC3 lipidation and it is linked to early autophagosomal structures together with Atg16L and ULK1.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
WIPI-2 belongs to the WIPI subfamily of WD40 repeat proteins. It is widely expressed in a variety of cell lines. The protein is characterized with a 7-bladed propeller structure and contain a conserved motif for interaction with phospholipids. It is a membrane constituent of autophagosomes and the plasma membrane.
Immunogen
synthetic peptide corresponding to the C-terminal region of human WIPI-2, conjugated to KLH. The corresponding sequence is identical in mouse and rat.
Physical form
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Differential requirement for ATG2A domains for localization to autophagic membranes and lipid droplets
Tamura N, et al.
Febs Letters, 591(23), 3819-3830 (2017)
Autophagosomal YKT6 is required for fusion with lysosomes independently of syntaxin 17
Matsui T, et al.
The Journal of cell biology, 217(8), 2633-2645 (2018)
Kenta Imai et al.
Journal of cell science, 129(20), 3781-3791 (2016-09-03)
Autophagy is an intracellular degradation pathway conserved in eukaryotes. Among core autophagy-related (Atg) proteins, mammalian Atg9A is the sole multi-spanning transmembrane protein, and both of its N- and C-terminal domains are exposed to the cytoplasm. It is known that Atg9A
Freeze-fracture replica immunolabelling reveals human WIPI-1 and WIPI-2 as membrane proteins of autophagosomes.
Proikas-Cezanne T
Journal of Cellular and Molecular Medicine, 15(9), 2007-2010 (2011)
Mammalian Atg18 (WIPI2) localizes to omegasome-anchored phagophores and positively regulates LC3 lipidation.
Polson HE
Autophagy, 6(4), 506-522 (2010)
相关内容
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