SAB4200369
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse
clone R2.5254.2, purified from hybridoma cell culture
别名:
Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)
登录查看公司和协议定价
所有图片(2)
About This Item
UNSPSC代码:
12352203
NACRES:
NA.41
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
R2.5254.2, monoclonal
形式
buffered aqueous solution
分子量
antigen ~72 kDa
种属反应性
rat, human, monkey, mouse
浓度
~1.0 mg/mL
技术
flow cytometry: 5-10 μg/test using using 1x106 MDA-MB-231 cells
immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells
indirect ELISA: suitable
western blot: suitable
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... NECTIN2(5819) , PVRL2(5819) , PVRL2(308417)
mouse ... Nectin2(19294)
相关类别
一般描述
Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.
Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.
特异性
The antibody has been reported to block cell adhesion and herpes virus entry.
免疫原
3T3 cells expressing human Nectin-2.
应用
Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunoblotting
- immunocytochemistry
- flow cytometry
生化/生理作用
Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our 产品选型工具.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Viral entry mechanisms: cellular and viral mediators of herpes simplex virus entry.
Akhtar J and Shukla D
FEBS Journal, 276, 7228-7236 (2009)
Serum nectin-2 levels are diagnostic and prognostic in patients with colorectal carcinoma.
Karabulut M
Clinical And Translational Oncology, 18, 160-171 (2016)
The cell adhesion molecule nectin-1 is critical for normal enamel formation in mice.
Barron MJ
Human Molecular Genetics, 17, 3509-3520 (2008)
PVRL2 is translocated to the TRA@ locus in t(14;19)(q11;q13)-positive peripheral T-cell lymphomas.
Almire C
Genes Chromosomes Cancer, 46, 1011-1018 (2007)
Sequencing of 42kb of the APO E-C2 gene cluster reveals a new gene: PEREC1.
Dna Sequence, 9, 89-100 (1998)
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门