SAB4200366
Anti-IKZF1/IKAROS antibody, Mouse monoclonal
clone E1.14, purified from hybridoma cell culture
别名:
Anti-Hs.54452, Anti-IK1, Anti-IKAROS family zinc finger 1, Anti-LYF1, Anti-PRO0758, Anti-ZNFN1A1, Anti-hIk-1
产品名称
Anti-IKZF1/IKAROS antibody, Mouse monoclonal, clone E1.14, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
E1.14, monoclonal
form
buffered aqueous solution
mol wt
antigen ~55 kDa
species reactivity
rat, monkey, human, mouse
concentration
~1.0 mg/mL
technique(s)
flow cytometry: suitable
immunocytochemistry: 0.25-0.5 μg/mL using Jurkat cells
indirect immunofluorescence: suitable
western blot: 0.25-0.5 μg/mL using Ramos cells
isotype
IgG2b
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IKZF1(10320)
Application
Monoclonal Anti-IKZF1 (Ikaros family zinc finger protein 1)/IKAROS antibody produced in mouse has been used in:
- immunoblotting
- immunofluorescence
- immunohistochemistry
- flow cytometry
Biochem/physiol Actions
Ikaros family zinc finger protein 1 (IKZF1)/IKAROS activity is essential for the generation of B cell precursors. Ikaros is essential at the earliest stage of T cell and B cell specification. In adults, however, lymphoid lineages rely on Ikaros at distinct phases of their development. Mutations or polymorphisms are associated with human B-cell acute lymphoblastic leukemia (B-ALL). Ikaros functions as a tumor suppressor in T-cell acute lymphoblastic leukemia (T-ALL) and B-ALL. Ikaros deletion was detected in patients with blast-phase myeloproliferative neoplasms.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti- IKZF1 (Ikaros family zinc finger protein 1)/IKAROS (mouse IgG2b isotype) is derived from the hybridoma E1.14 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a peptide located at the C-terminus human IKZF1/IKAROS. The IKAROS gene encodes a family of hemopoietic specific zinc finger transcriptional regulator proteins, which binds to specific DNA target sequences consisting of TGGGAA consensus motif.
Immunogen
a peptide located at the C-terminus human IKZF1/IKAROS.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Role of Ikaros in T-cell acute lymphoblastic leukemia
Kastner P and Chan S
World Journal of Biological Chemistry, 2(6), 108-108 (2011)
Ikaros is critical for B cell differentiation and function
Kirstetter P, et al.
European Journal of Immunology, 32(3), 720-730 (2002)
Novel mutations and their functional and clinical relevance in myeloproliferative neoplasms: JAK2, MPL, TET2, ASXL1, CBL, IDH and IKZF1
Tefferi A
Leukemia, 24(6), 1128-1128 (2010)
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