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安全信息

SAB4200365

Sigma-Aldrich

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

别名:

Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

抗体产品类型

primary antibodies

克隆

PIWIL1-2, monoclonal

表单

buffered aqueous solution

分子量

antigen ~95 kDa

种属反应性

human, mouse

浓度

~1.0 mg/mL

技术

immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin® Peroxidase staining system.
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.

同位素/亚型

IgG2b

UniProt登记号

Q96J94

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... PIWIL1(9271)
mouse ... Piwil1(57749)

一般描述

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

免疫原

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:
  • immunofluorescence
  • immunoprecipitation
  • flow cytometry analysis29}
  • immunoblotting
  • immunohistochemistry

生化/生理作用

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

法律信息

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

10 - Combustible liquids

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000304839
0000304839
0000253725
0000249546
0000238064

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Molecular characterization of hiwi, a human member of the piwi gene family whose overexpression is correlated to seminomas
Qiao D, et al.
Oncogene, 21(25), 3988-3988 (2002)
An Lnc RNA (GAS5)/SnoRNA-derived piRNA induces activation of TRAIL gene by site-specifically recruiting MLL/COMPASS-like complexes
He X, et al.
Nucleic Acids Research, 43(7), 3712-3725 (2015)
High levels of PIWI-interacting RNAs are present in the small RNA landscape of prostate epithelium from vitamin D clinical trial specimens
Baumann B, et al.
Prostate, 79(8), 840-855 (2019)
The biogenesis and function of PIWI proteins and piRNAs: progress and prospect
Thomson T and Lin H
Annual Review of Cell and Developmental Biology, 25, 355-376 (2009)
Xue Zhang et al.
Journal of immunology (Baltimore, Md. : 1950), 196(4), 1591-1603 (2016-01-13)
The discovery of PIWI-interacting RNAs (piRNAs) revealed the complexity of the RNA world. Although piRNAs were first deemed to be germline specific, substantial evidence shows their various roles in somatic cells; however, their function in highly differentiated immune cells remains
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