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生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
KLF5-55, monoclonal
形式
buffered aqueous solution
分子量
antigen ~55
种属反应性
rat, mouse, chicken, human, hamster, canine, monkey
浓度
~1.0 mg/mL
技术
indirect immunofluorescence: 2.5-5.0 μg/mL using methanol/acetone fixed PC3 cells.
western blot: 1.0-2.0 μg/mL using extracts of PC3 cells.
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... KLF5(688)
mouse ... Klf5(12224)
rat ... Klf5(84410)
一般描述
Kruppel like factor 5 (KLF5) is encoded by the gene mapped to human chromosome 13q22.1. The encoded protein belongs to kruppel-like factor (KLF) protein family and is mainly expressed in variety of tissues, including lung, colon, intestine and pancreas.
Monoclonal Anti-KLF5 (mouse IgG1 isotype) is derived from the hybridoma KLF-55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. KLF5 is a DNA-binding transcriptional regulator. This protein contains three independent peptide modules of the C2H2 zinc finger-type. KLF5 protein is expressed in the prostate, kidney, skeletal muscle, and breast. KLF5 gene is also expressed in embryos and is highly expressed in the later stage of fetal development.
特异性
Monoclonal Anti-KLF5 recognizes human, monkey, dog, hamster, mouse and rat KLF5.
免疫原
synthetic peptide corresponding to a sequence at the C-terminus of human KLF5, conjugated to KLH. The corresponding sequence is identical to monkey, bovine, chicken, rat and mouse KLF5.
应用
Anti-KLF5 antibody, Mouse monoclonal has been used in western blotting and immunofluorescence.
生化/生理作用
KLF5 protein functions through the restoration of cyclin D1, cyclin B1 and Cdc2 gene expression. This protein is also involved in regulating the inhibitory effect of retinoids on cell proliferation. KLF5 protein regulates the pro-proliferative and transforming activities of oncogenic H-ras. KLF5 protein plays a role as a tumor suppressor in intestinal tumorigenesis in vivo. Deletion and down-regulation of KLF5 leads to prostate and breast cancer.
Kruppel like factor 5 (KLF5) has been involved in the regulation of various cellular functions, such as cell cycle, apoptosis, proliferation and differentiation, in both normal and tumor cells. Additionally, it also plays a vital role in maintenance of cardiovascular remodeling. Overexpression of the gene has been observed in various cancer types, including cervical cancer(CC) and colon cancer. Hence, this can be used as a prognostic marker for these cancers.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
For continuous use, store at 2-8°C for up to one month. For extended storage, freeze at -20oC in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Characterization of the expression profiles of adipogenesis-related factors, ZNF423, KLFs and FGF10, during preadipocyte differentiation and abdominal adipose tissue development in chickens
Matsubara Y
Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 165, 189-195 (2013)
FBW7-mediated ubiquitination and degradation of KLF5
Yi Luan
World Journal of Biological Chemistry, 5, 216-223 (2014)
KLF5 promotes hypoxia-induced survival and inhibits apoptosis in non-small cell lung cancer cells via HIF-1?
Li X
International Journal of Oncology, 45, 1507-1514 (2014)
Dan Tong et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 12(8), 2442-2448 (2006-04-28)
Kruppel-like factor (KLF5) is a cell growth mediator in various epithelial cells. Higher KLF5 increases cell growth rate and leads to transformed phenotypes. Because tumor cell proliferation is tightly associated with tumor progression, and consequently, with survival of cancer patients
Weishi Wang et al.
Poultry science, 96(1), 200-210 (2016-09-03)
Gga-miR-21 is abundantly expressed in chicken pre-adipocytes, but its role is unclear. The present study investigated the role of gga-miR-21 in chicken pre-adipocyte proliferation. Cell proliferation assay and gene expression analysis of proliferating cell nuclear antigen (PCNA) showed that the
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