推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
种属反应性
mouse
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 1-2 μg using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
western blot: 0.5-1 μg/mL using lysates of HEK-293T cells co-expressing mCD3ϲ and constitutively active LCK.
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
phosphorylation (pTyr142)
基因信息
human ... CD247(919)
mouse ... Cd247(12503)
一般描述
The TCR-CD3 multimeric complex is composed of TCRαβ, CD3γε and CD3δε and TCR‐ζ homodimer. The CD3γ, CD3δ and CD3ε, each contain one immunoreceptor tyrosine-based activation motif (ITAM), whereas the ζ chain contains three ITAMs.
免疫原
synthetic phosphopeptide corresponding to residues surrounding Tyr142 of mouse CD3ζ, conjugated to KLH. The corresponding sequence is identical in human CD3ζ.
应用
Anti-phospho-CD3ζ (pTyr142) antibody produced in rabbit has been used in several immunochemical techniques including immunoblotting and immunoprecipitation.
生化/生理作用
Amongst the TCR subunits, the CD3 ζ chain plays a key role in regulating TCR assembly, transport, and cell surface expression. Down-regulation of TCR ζ chain expression associated with T cell dysfunction was described in various pathologies.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment
Kim K D, et al.
Nature Communications, 8, 1-10 (2017)
TCR zeta-chain downregulation: curtailing an excessive inflammatory immune response
Baniyash M.
Nature Reviews: Immunology, 4, 675-687 (2004)
An architectural perspective on signaling by the pre?, ?? and ?? T cell receptors
Hayes SM, et al.
Immunological Reviews, 191, 28-37 (2003)
Function of Cd3?-Mediated Signals in T Cell Development
Sommers C. L.
The Journal of Experimental Medicine, 192, 913-913 (2000)
Kyun-Do Kim et al.
Nature communications, 8, 15365-15365 (2017-05-16)
Adoptive cell transfer utilizing tumour-targeting cytotoxic T lymphocytes (CTLs) is one of the most effective immunotherapies against haematological malignancies, but significant clinical success has not yet been achieved in solid tumours due in part to the strong immunosuppressive tumour microenvironment.
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