产品名称
Anti-Cullin 3 antibody ,Mouse monoclonal, clone CUL3-9, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CUL3-9, monoclonal
form
buffered aqueous solution
mol wt
antigen 80 kDa
species reactivity
rat, human, mouse
concentration
~1.0 mg/mL
technique(s)
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using whole extracts of human Jurkat cells or mouse brain.
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CUL3(8452)
mouse ... Cul3(26554)
rat ... Cul3(301555)
Application
Monoclonal Anti-Cullin 3 antibody produced in mouse has been used in western blotting and immunoprecipitation.
Biochem/physiol Actions
Cullin 3 (CUL3) acts as a scaffold protein and facilitates the organization of a large number of ubiquitin ligase complexes. It plays a vital role in the regulation of electrolyte homeostasis and blood pressure. The encoded protein acts as a key factor in protein ubiquitination and is also implicated in early embryonic development. One important role of CUL3 is to promote invasion and migration of trophoblast cells. Mutation in the gene leads to onset of pre-eclampsia and pseudohypoaldosteronism type II.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Cullin 3 (CUL3) is a member of cullin protein family and is encoded by the gene mapped to human chromosome 2q36.2. The encoded protein is a core component of cullin/RING E3 ubiquitin ligase (CRL) and is ubiquitously expressed. The protein is characterized with a cullin homology domain.
Monoclonal Anti-Cullin 3 (mouse IgG1 isotype) is derived from the hybridoma CUL3-9 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide of human Cullin 3.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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存储类别
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Defining the human sperm microtubulome: an integrated genomics approach
Jumeau F, et al.
Biology of Reproduction, 96(1), 93-106 (2016)
Mutations in Kelch-like 3 and Cullin 3 cause hypertension and electrolyte abnormalities
Boyden LM
Nature, 482, 98-102 (2012)
Neddylated Cullin 3 is required for vascular endothelial-cadherin-mediated endothelial barrier function
Sakaue T, et al.
Cancer Science, 108(2), 208-215 (2017)
The CUL3-SPOP-DAXX axis is a novel regulator of VEGFR2 expression in vascular endothelial cells
Sakaue T, et al.
Scientific reports, 7(2), 42845-42845 (2017)
Cullin3-BTB interface: a novel target for stapled peptides.
de Paola I
PLoS ONE, 10 (2015)
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