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安全信息

SAB4200141

Sigma-Aldrich

Anti-Rictor antibody produced in rabbit

~1-2 μg/mL, affinity isolated antibody

别名:

ANTI-Pianissimo, ANTI-Rapamycin-insensitive companion of mTOR, ANTI-TORC2-specific protein AVO3, Anti-RPTOR independent companion of MTOR, complex 2, AVO3

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.44

生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

抗体产品类型

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

分子量

~190 kDa

种属反应性

human, rat, mouse

包装

antibody small pack of 25 μL

浓度

~1-2 μg/mL

技术

immunohistochemistry: 5-10 μg/mL using a working concentration of 5-10 ug/mL is recommended using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin / ExtrAvidin Peroxidase staining system.
immunoprecipitation (IP): 5-10 μg using a working amount of 5-10 μg is recommended using lysates of human HeLa cells.
western blot: 1-2 μg/mL using a working concentration of 1-2 μg/mL is recommended using whole extracts of mouse C2C12 cells.

UniProt登记号

Q6R327

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... RICTOR(253260)
mouse ... rictor(78757)
rat ... rictor(310131)

一般描述

Rictor (rapamycin-insensitive companion of mTOR) is a subunit of the mammalian target of rapamycin complex 2 (mTORC2). The mTORC2 complex contains mTOR, mLST8, mSIN1, deptor, protor-1 and rictor. mTOR substrate that regulates cell size.
m-TOR is a conserved kinase that primarily regulates cell growth. Rictor is a rapamycin-insensitive companion of m-TOR and regulates the phosphorylation of Akt/PKB. Rictor also phosphorylates PKCα and actin cytoskeleton .

特异性

Anti-Rictor antibody is specific for human, mouse and rat rictor. In immunoblotting, detection of the rictor band (approx. 190 kDa) is specifically inhibited by the immunizing peptide.

免疫原

The corresponding sequence is identical in mouse and rat rictor.

应用

Anti-Rictor antibody is suitable for use in immunohistochemistry (5-10 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded rat testis sections and biotin/ExtrAvidin Peroxidase staining system). The product may also be used for immunoprecipitation (5-10 μg using lysates of human HeLa cells), and immunoblot (1-2 μg/mL is using whole extracts of mouse C2C12 cells).

生化/生理作用

Rictor (rapamycin-insensitive companion of mTOR) regulates cell survival and cytoskeletal organization through the regulation of Akt/ protein kinase B (PKB) and protein kinase C alpha (PKCα).

外形

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Targeting mTORC2 inhibits colon cancer cell proliferation in vitro and tumor formation in vivo
Roulin D, et al.
Molecular Cancer, 9(1), 57-57 (2010)
Estela Jacinto et al.
Nature cell biology, 6(11), 1122-1128 (2004-10-07)
The target of rapamycin (TOR) is a highly conserved protein kinase and a central controller of cell growth. In budding yeast, TOR is found in structurally and functionally distinct protein complexes: TORC1 and TORC2. A mammalian counterpart of TORC1 (mTORC1)
mTORC2 activity is elevated in gliomas and promotes growth and cell motility via overexpression of rictor
Masri J, et al.
Cancer research, 67(24), 11712-11720 (2007)
D D Sarbassov et al.
Current biology : CB, 14(14), 1296-1302 (2004-07-23)
The mammalian TOR (mTOR) pathway integrates nutrient- and growth factor-derived signals to regulate growth, the process whereby cells accumulate mass and increase in size. mTOR is a large protein kinase and the target of rapamycin, an immunosuppressant that also blocks
Tianyu Hang et al.
International journal of molecular sciences, 24(10) (2023-05-27)
Type-2 diabetes (T2DM) and arterial hypertension (HTN) are major risk factors for heart failure. Importantly, these pathologies could induce synergetic alterations in the heart, and the discovery of key common molecular signaling may suggest new targets for therapy. Intraoperative cardiac
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