生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen ~65 kDa
种属反应性
human, rat, mouse
浓度
~1.0 mg/mL
技术
western blot: 1-2 μg/mL using whole extracts of human HeLa cells and 0.5-1 μg/mL using whole extracts of mouse 3T3 or rat PC12 cells.
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... KIAA0652(9776)
一般描述
Autophagy-related protein 13 (Atg13) is mapped to human chromosome 11p11.2. It comprises of HORMA domain (Hop1, Rev7 and MAD2).
特异性
Anti-Atg13 recognizes human, mouse, and rat Atg13.
应用
Anti-ATG13 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.
生化/生理作用
Autophagy-related protein 13 (Atg13) is essential for autophagosome formation in mammalian cells. Atg13 forms a stable complex with ULK1 (Unc-51 Like Autophagy Activating Kinase 1) and FAK family-interacting protein of 200 kDa (FIP200). Mammalian target of rapamycin (mTOR) interacts with this complex in a nutrient dependent manner and phosphorylates Atg13 and ULK1, suggesting that mTOR regulates autophagy through the ULK1-Atg13-FIP200 complex.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
Wei Lu et al.
Stem cells and development, 24(9), 1133-1140 (2014-12-02)
The 3' untranslated region (UTR)-associated RNAs (uaRNAs) have important roles in various biological processes, especially in development. However, since they overlap with protein-coding mRNAs, uaRNAs are difficult to study by RNA interference techniques. We recently identified a chemical molecule, 3-benzyl-5-((2-nitrophenoxy)
Physical and neurobehavioral determinants of reproductive onset and success
Day FR, et al.
Nature Genetics, 48(6), 617-617 (2016)
Autophagosome formation is initiated at phosphatidylinositol synthase-enriched ER subdomains
Nishimura T, et al.
The Embo Journal, 36(12), 1719-1735 (2017)
Di Ge et al.
Autophagy, 10(6), 957-971 (2014-06-01)
MTOR, a central regulator of autophagy, is involved in cancer and cardiovascular and neurological diseases. Modulating the MTOR signaling balance could be of great significance for numerous diseases. No chemical activators of MTOR have been found, and the urgent challenge
ULK-Atg13-FIP200 complexes mediate mTOR signaling to the autophagy machinery
Jung CW, et al.
Molecular Biology of the Cell, 20(7), 1992-2003 (2009)
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