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![Anti-phospho-TDP-43 [pSer409] antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody](/deepweb/assets/sigmaaldrich/product/images/407/478/bcd6d544-d187-42ed-8c1e-98bf709b4da7/640/bcd6d544-d187-42ed-8c1e-98bf709b4da7.jpg)
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~60 kDa
种属反应性
human, rat, mouse
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1.5 mg/mL
技术
immunocytochemistry: 5-10 μg/mL using HeLa cells
immunohistochemistry: 20-30 μg/mL using formalin-fixed paraffin embedded human colon
immunoprecipitation (IP): 2-4 μg using rat brain extract (S2 fraction)
western blot: 1-2 μg/mL using mouse brain extract (S2 fraction)
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PKM2(5315)
一般描述
丙酮酸激酶（PK）是糖酵解的关键调节酶，具有四种同种型：L、R、M1和M2。同种型M2与癌转移有关。PK 同种型 M1 在胚胎发育过程中表达，是心脏、脑和骨骼肌组织中的主要同种型。
特异性
抗 PKM2 （同种型M1） 抗体对人类、小鼠和大鼠 PKM2（同种型M1）/PKM1 有特异性。在免疫印迹中，PKM2（同种型M1）/PKM1条带的检测被免疫肽特异性抑制。
应用
PKM2（同种型M1）抗体还可用于：
- 免疫印迹
- 免疫沉淀
- 免疫组化
- 免疫细胞化学
生化/生理作用
丙酮酸激酶（PK） 是糖酵解途径中的关键酶。研究证明，人类癌细胞系中M2 同种型的敲低及其被 M1 同种型替代会导致 Warburg 效应的逆转，并降低在小鼠异种移植物中形成肿瘤的能力。M2 同种型Tyr105 处 的磷酸化会抑制其活性，并且在人类癌症中很常见，这表明 Tyr105 是癌细胞中促进肿瘤发生的关键代谢开关（metabolic switch）。
外形
0.01M磷酸盐缓冲盐水溶液，pH 7.4，含有15 mM叠氮化钠。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的人类或动物食用或应用。
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Mohammed Alquraishi et al.
Cell communication and signaling : CCS, 20(1), 76-76 (2022-06-01)
Acute kidney injury (AKI) is associated with a severe decline in kidney function caused by abnormalities within the podocytes' glomerular matrix. Recently, AKI has been linked to alterations in glycolysis and the activity of glycolytic enzymes, including pyruvate kinase M2
Ataxin-1 regulates the cerebellar bioenergetics proteome through the GSK3beta-mTOR pathway which is altered in Spinocerebellar ataxia type 1 (SCA1)
Sanchez I, et al.
Human Molecular Genetics, 25(18), 4021-4040 (2016)
Zhitao Chen et al.
Oncology reports, 50(5) (2023-09-21)
In the present study, it was aimed to investigate the effects and potential mechanisms of heat shock protein B7 (HSPB7) on lung adenocarcinoma (LUAD). Bioinformatic analysis was performed to explore the association between HSPB7 expression and patients with LUAD. MTT
Seung-Yon Lee et al.
The Journal of clinical investigation, 128(12), 5573-5586 (2018-10-05)
Notch signaling critically controls cell fate decisions in mammals, both during embryogenesis and in adults. In the skeleton, Notch suppresses osteoblast differentiation and sustains bone marrow mesenchymal progenitors during postnatal life. Stabilizing mutations of Notch2 cause Hajdu-Cheney syndrome, which is
Shawn M Davidson et al.
Cancer research, 82(13), 2403-2416 (2022-05-19)
Differential expression of PKM1 and PKM2 impacts prostate tumorigenesis and suggests a potential therapeutic vulnerability in prostate cancer.
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