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生物来源
mouse
偶联物
unconjugated
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
8B3-C3-E4, monoclonal
形式
buffered aqueous solution
分子量
antigen ~236 kDa
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~1.0 mg/mL
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 3.0-6.0 μg/mL using MDA-MB-231 cell extracts
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... DOCK9(23348)
一般描述
Monoclonal Anti-DOCK9 (Zizimin1) (mouse IgG2a isotype) is derived from the hybridoma 8B3-C3-E4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Zizimin1 is a guanine nucleotide exchange factor (GEF) specific to Cdc42. It is a part of the second family of mammalian Rho-GEFs, CZH [CDM (Ced-5/DOCK180/Myoblast city)-zizimin homology] proteins. The Zizimin1 protein contains the CZH2 domain, CZH1 domain and Pleckstrin homology (PH) domain.
特异性
Monoclonal Anti-DOCK9 (Zizimin1) recognizes human DOCK9.
免疫原
full length DOCK9 (Zizimin1)
应用
Anti-DOCK9 (Zizimin 1) antibody, Mouse monoclonal is suitable for:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
生化/生理作用
Zizimin1/ dedicator of cytokinesis 9 (DOCK9) is found to activate Cdc42 through binding to its CZH2 domain. Interestingly, the CZH1 domain of Zizimin1 at the N-terminal binds to its CZH2 domain and thus inhibits Cdc42 binding. The Pleckstrin homology (PH) domain at the N-terminal, binds phosphoinositides and results in zizimin1 membrane targeting.
外形
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
The novel Cdc42 guanine nucleotide exchange factor, zizimin1, dimerizes via the Cdc42-binding CZH2 domain
Meller N, et al.
Test, 279(36), 37470-37476 (2004)
Function of the N-terminus of zizimin1: autoinhibition and membrane targeting
Meller N, et al.
The Biochemical Journal, 409(2), 525-533 (2008)
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