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生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~77 kDa
浓度
~1.0 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using lysates of HEK-293T cells
indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HEK-293T cells
western blot: 1-2 μg/mL using lysates of K562 cells
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... RAVER1(125950)
mouse ... Raver1(71766)
rat ... Raver1(298705)
一般描述
The protein ribonucleoprotein, PTB binding 1 (Raver1), also known as ribonucleoprotein polypyrimidine tract-binding (PTB) protein 1, is a widely expressed multidomain protein. Raver1 is composed of three RNA recognition motifs (RRM) and of nuclear localization and nuclear export signals, allowing it to shuttle between the nucleus and the cytoplasm. The RAVER1 gene is mapped to human chromosome 19p13.2.
特异性
Anti-Raver1 (N-terminal) recognizes human Raver1.
应用
Anti-Raver1 (N-terminal) antibody produced in rabbit is suitable for:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
The protein ribonucleoprotein, PTB binding 1 (Raver1) is identified in two-hybrid screens by its ability to interact and colocalize with the cytoskeletal proteins α-actinin and vinculin. In skeletal muscle, the shuttling of Raver1 from nucleus to cytoplasm is associated with the differentiation of specific microfilament attachment sites. Based on an analysis of vinculin:Raver1 crystal structure, it was suggested that vinculin recruits Raver1 and its mRNAs cargo to focal adhesions, promoting restricted synthesis of adhesion complexes by the translational machinery.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 °C. For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Functional characterization of the ribonucleoprotein, PTB-binding 1/Raver1 promoter region
Romanelli MG, et al.
Gene, 405(1-2), 79-87 (2007)
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins
Hu?ttelmaier S, et al.
Test, 155(5), 775-786 (2001)
The metavinculin tail domain directs constitutive interactions with raver1 and vinculin RNA
Lee JH, et al.
Journal of Molecular Biology, 422(5), 697-704 (2012)
Raver1 interactions with vinculin and RNA suggest a feed-forward pathway in directing mRNA to focal adhesions
Lee JH, et al.
Structure, 17(6), 833-842 (2009)
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