推荐产品
生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~82 kDa
种属反应性
rat, mouse, human
包装
antibody small pack of 25 μL
浓度
~1.5 mg/mL
技术
immunoprecipitation (IP): 10-15 μg using A431 cell lysates
indirect immunofluorescence: 8-16 μg/mL using HeLa cells
western blot: 1-2 μg/mL using extracts of mouse brain (S1 fraction) or rat brain (S1 fraction)
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... VAC14(55697)
rat ... Vac14(307842)
一般描述
VAC14 gene is mapped to human chromosome 16q22.1-q22.2. VAC14 is a scaffold protein and is a component of the PIKfyve protein kinase complex.
特异性
Anti-VAC14 (C-terminal) specifically recognizes human, mouse, and rat VAC14.
应用
Anti-VAC14 (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
Mutation in VAC14 gene leads to the development of retinitis pigmentosa (RP).
VAC14 is a regulator of PIP5K3/PIKfyve, a dual specificity kinase.
VAC14 phosphorylates PtdIns(3)P to generate the housekeeping phospholipid PtdIns(3,5)P2. It associates with and upregulate PIKfyve phosphoinositide-5-kinase activity. VAC14-PIP5K3- PtdIns(3,5)P2 pathway is thought to be physiologically linked to insulin activation of glucose transport into the cell. Knockout of the VAC14 gene in mice results in massive neurodegeneration and particularly affected are neurons in the midbrain and of the peripheral sensory system.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
储存及稳定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Gholson J Lyon et al.
Cold Spring Harbor molecular case studies, 5(6) (2019-08-08)
Whole-exome sequencing was used to identify the genetic etiology of a rapidly progressing neurological disease present in two of six siblings with early childhood onset of severe progressive spastic paraparesis and learning disabilities. A homozygous mutation (c.2005G>T, p, V669L) was
ArPIKfyve homomeric and heteromeric interactions scaffold PIKfyve and Sac3 in a complex to promote PIKfyve activity and functionality
Sbrissa D, et al.
Journal of Molecular Biology, 384(4), 766-779 (2008)
PIKfyve: Partners, significance, debates and paradoxes
Shisheva A
Cell Biology International, 32(6), 591-604 (2008)
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