SAB4200053
Anti-DNA polymerase δ p125 antibody, Rat monoclonal
clone PDG 5G1, purified from hybridoma cell culture
别名:
Anti-POLD1, Anti-Polymerase (DNA directed), delta 1, catalytic subunit 125kDa, Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat
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所有图片(1)
About This Item
UNSPSC代码:
12352203
NACRES:
NA.41
推荐产品
生物来源
rat
质量水平
抗体形式
purified from hybridoma cell culture
抗体产品类型
primary antibodies
克隆
PDG 5G1, monoclonal
表单
buffered aqueous solution
分子量
antigen ~125 kDa
种属反应性
human, mouse, rat
包装
antibody small pack of 25 μL
技术
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: 0.5-1.0 μg/mL using Jurkat cells extract
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... POLD1(5424)
一般描述
Monoclonal Anti-DNA polymerase d p125 (rat IgG2a isotype) is derived from the hybridoma PDG 5G1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with human p125. The catalytic subunit p125 (POLD1), which is encoded by the Pold1 gene, is highly conserved among eukaryotes. Pold1 comprises of two primary functional domains: an exonuclease domain near the N-terminus and the subsequent polymerase domain.
免疫原
human p125
应用
Monoclonal Anti-DNA polymerase δ p125 antibody produced in rat has been used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- co-immunoprecipitation
生化/生理作用
The exonuclease domain of the catalytic subunit p125 (POLD1) has the ability to catalyze 3′−5′ exonucleolytic proofreading and the polymerase domain helps to catalyze DNA synthesis. Mutations in this subunit have been implicated in mammalian embryogenesis and tumorigenesis.
外形
Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Human DNA polymerase delta is a pentameric holoenzyme with a dimeric p12 subunit
Khandagale P, et al.
Life science alliance, 2(2) (2019)
Eukaryotic DNA polymerases, a growing family
Huubscher U, et al.
Trends in Biochemical Sciences, 25(3), 143-147 (2000)
DNA Polymerase delta Is Required for Early Mammalian Embryogenesis
Uchimura A, et al.
Testing, 4(1) (2009)
Prashant Khandagale et al.
Life science alliance, 2(2) (2019-03-20)
Human DNA polymerase delta (Polδ), a holoenzyme consisting of p125, p50, p68, and p12 subunits, plays an essential role in DNA replication, repair, and recombination. Herein, using multiple physicochemical and cellular approaches, we found that the p12 protein forms a
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