Anti-dUTPase antibody, Rat monoclonal

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) maintains the genomic integrity of cells. This enzyme is a symmetrical trimer with 152 amino acids.

Monoclonal Anti-dUTPase (rat IgG2a isotype) is derived from the hybridoma HUTP 3E6 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from a rat immunized with protein. The DUT gene is mapped on the human chromosome at 15q21.1.

Monoclonal Anti-dUTPase recognizes human, mouse and marmoset dUTPase.

human dUTPase protein.

Monoclonal Anti-dUTPase antibody produced in rat may be used in:

• immunoblotting
• immunoprecipitation
• immunocytochemistry

Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) hydrolyses dUTP to dUMP and pyrophosphate and promotes nucleotide metabolism. Epstein-Barr virus (EBV) encoded dUTP plays a crucial role in pathophysiology of EBV related diseases, by regulating cellular microenvironment. It is used as a target for anticancer chemotherapy.

Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death.

Solution in 0.01M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Store at -20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.