Anti-MLPH antibody produced in mouse

MLPH (melanophilin) belongs to the family of synaptotagmin-like proteins and is a Ras-related protein Rab27A effector. It is a component of a tripartite complex, which also includes myosin-Va and Rab27A. The protein contains a Slp homology domain (SHD) in its N-terminal and a myosin Va-binding region in its C-terminal. Also, amino acids 400-590 in the C-terminal, directly bind to actin. MLPH contains two α-helices (SHD1 and SHD2), which are conserved in nature. These two regions are intervened by two zinc finger motifs. The MLPH gene is localized to human chromosome 2q37.3.

MLPH (NP_077006.1, 1 a.a. ~ 600 a.a) full-length human protein.

Sequence
MGKKLDLSKLTDEEAQHVLEVVQRDFDLRRKEEERLEALKGKIKKESSKRELLSDTAHLNETHCARCLQPYQLLVNSKRQCLECGLFTCKSCGRVHPEEQGWICDPCHLARVVKIGSLEWYYEHVKARFKRFGSAKVIRSLHGRLQGGAGPELISEERSGDSDQTDEDGEPGSEAQAQAQPFGSKKKRLLSVHDFDFEGDSDDSTQPQGHSLHLSSVPEARDSPQSLTDESCSEKAAPHKAEGLEEADTGASGCHSHPEEQPTSISPSRHGALAELCPPGGSHRMALGTAAALGSNVIRNEQLPLQYLADVDTSDEESIRAHVMASHHSKRRGRASSESQIFELNKHISAVECLLTYLENTVVPPLAKGLGAGVRTEADVEEEALRRKLEELTSNVSDQETSSEEEEAKDEKAEPNRDKSVGPLPQADPEVGTAAHQTNRQEKSPQDPGDPVQYNRTTDEELSELEDRVAVTASEVQQAESEVSDIESRIAALRAAGLTVKPSGKPRRKSNLPIFLPRVAGKLGKRPEDPNADPSSEAKAMAVPYLLRRKFSNSLKSQGKDDDSFDRKSVYRGSLTQRNPNARKGMASHTFAKPVVAHQS

MLPH (melanophilin) acts as a receptor for myosin-Va in melanocytes. It is essential for the transport of melanosomes from perinuclear region to the actin-rich periphery of melanocytes. Thus, normal melanosome distribution is dependent on this protein. MLPH acts as a linker between myosin-Va and Rab27A, and facilitates the binding of melanosomes with actin network. This protein might also be a key player in the instantaneous skin tanning in humans, when exposed to sun. Mutation in MLPH gene leads to mice with light coat color, called leaden mice. It is the outcome of aberrant melanosome transportation. Homozygous missense mutation occurring in the Slp homology domain of this protein is associated with Griscelli syndrome type III (GSIII). MLPH is under-expressed in GSIII melanocytes and such patients are characterized by silvery-gray hair and hypopigmentation of the skin.

Solution in phosphate buffered saline, pH 7.4