S9555
Anti-E-Selectin (CD62E) antibody, Mouse monoclonal
clone 1.2B6, purified from hybridoma cell culture
别名:
Monoclonal Anti-CD62E, Anti-E-Selectin, Anti-ELAM-1
产品名称
Anti-E-Selectin (CD62E) antibody, Mouse monoclonal, clone 1.2B6, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1.2B6, monoclonal
form
buffered aqueous solution
mol wt
antigen 100-115 kDa
species reactivity
human, pig
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable
flow cytometry: suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1-2 μg/mL using using 293-T cells transfected with E-selectin fusion protein
western blot: suitable (non-reduced)
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SELE(6401)
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Application
Anti-E-Selectin (CD62E) antibody, Mouse monoclonal has been used in immunofluorescence.
Biochem/physiol Actions
E-selectin acts as a key inflammatory cytokine. E-selectin-dependent rolling helps in binding pancreatic cancer cell to hyaluronic acid. In addition, the encoded protein in association with P-selectins, plays a vital role in recruiting leukocytes to inflammatory sites. Mutation in the gene is associated with the development of various diseases such as atherosclerosis and stroke.
Reacts specifically with human CD62E (E-Selectin, ELAM-1) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant hman P-Selectin and human platelet P-Selectin. Cross-reactivity has been observed with porcine E-Selectin. Monoclonal Anti-human E-Selctin inhibits adhesion of neutrophils, eosinophils, and a skin-homing T-cell subpopulation to activated endothelial cells and CD62E-transfected cells. 5th Workshop: code no. E 040.
Selectins play a vital role in maintaining homoeostasis and cutaneous inflammation. SEL-E and SEL-P help in carrying T helper (Th1) cells into inflamed tissues. SEL-E acts as a receptor for cluster of differentiation 44 (CD44 (HCELL)), Mac-2 binding protein, mucin 1 (Muc-1), death receptor-3 and E-selectin ligand-1 (ESL-1). Overexpression of the gene might affect adhesion and migration of the cells leading to the endometriosis disorder.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
CD62E (E-Selectin) surface antigen is a glycoprotein and member of the selectin family of adhesion receptors. It is expressed by activated human endothelial cells and is detectable in the plasma.
Monoclonal anti-E-selectin (CD62E) (mouse IgG1 isotype) is derived from the 1.2B6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with TNF-α activated human endothelial cells. This antibody reacts specifically with E-selectin (CD62E) expressed on the surface of activated endothelial cells. The antibody may weakly cross-react with recombinant human P-selectin and human platelet P-selectin. Cross-reactivity has been observed with porcine E-selectin.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.
It is a 140KDa protein mainly expressed in endothelial cells. E- selectin is characterized with an N-terminal domain, epidermal growth factor (EGF)-like domain, lectin (ligand-binding) domain, variable short consensus repeats and transmembrane domain.
Selectin E (SELE) is encoded by the gene mapped to human chromosome 1q24.2. The encoded protein is mainly expressed on luminal surfaces of inflamed vasculature.
Immunogen
TNF-α activated human endothelial cells.
Physical form
Solution, 0.2 μm filtered, in 0.01 M phosphate buffered saline, pH 7.4.
Preparation Note
For continuous use, store sterile at 2-8°C. For extended storage freeze in sterile working aliquots. Repeated freezing and thawing, or storage in "frostfree" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Effect of C1-INH on ischemia/reperfusion injury in a porcine limb ex vivo perfusion model.
Abdelhafez MM, et al.
Molecular Immunology, 88, 116-124 (2017)
Burcin Ekser et al.
Transplant international : official journal of the European Society for Organ Transplantation, 25(8), 882-896 (2012-05-31)
Upregulation of tissue factor (TF) expression on activated donor endothelial cells (ECs) triggered by the immune response (IR) has been considered the main initiator of consumptive coagulopathy (CC). In this study, we aimed to identify potential factors in the development
No association between E- and L-selectin genes and SLE: soluble L-selectin levels do correlate with genotype and a subset in SLE
Russell AI, et al.
Genes and Immunity, 6, 422-429 (2005)
E-selectin-mediated rolling facilitates pancreatic cancer cell adhesion to hyaluronic acid.
Shea DJ, et al.
Faseb Journal, 31, 5078-5086 (2017)
Association of E-selectin Gene Polymorphism (S128R) with Ischemic Stroke and Stroke Subtypes
Roy S, et al.
Inflammation, 37, 599-603 (2014)
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