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安全信息

S8820

Sigma-Aldrich

Protease Inhibitor Cocktail

SIGMA FAST, tablet, for the inhibition of serine, cysteine, aspartic and metalloproteases, For General Use

别名:

蛋白酶抑制剂片剂

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About This Item

UNSPSC代码:
12352200
NACRES:
NA.77

product name

SIGMAFAST™蛋白酶抑制剂片剂, For General Use

生物来源

bovine lung
bovine milk

质量水平

200

形式

tablet

溶解性

water: 1 tablet/100mL, clear to hazy, colorless

储存温度

2-8°C

相关类别

一般描述

SIGMAFAST™蛋白酶抑制剂片剂是由多种蛋白质抑制剂混合而成的水溶性片剂，包括AEBSF、E-64、bestatin、leupeptin、aprotinin和EDTA。 一片可以配制100mL酶复合液，并可抑制20 g细胞提取物中的蛋白酶。
SIGMAFAST蛋白酶抑制剂片剂不适合与HIS-Select®产品或其它固定化金属亲和色谱(IMAC)产品共同使用。该片剂中含有EDTA，它是一种金属螯合剂，可能会清除这些亲和产品中的金属离子。

特异性

抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶。

应用

SIGMAFAST蛋白酶抑制剂片剂的应用实例：
  • 洗涤公羊精液用于膜蛋白分析
  • 加入CD4+ T细胞孵育溶液，以获得总细胞裂解物用于蛋白质免疫印迹试验
  • 作为NP-40缓冲液组分，用于均质化位于损伤中心的脊髓组织以进行蛋白质印迹分析
  • 作为放射性免疫沉淀分析(RIPA)缓冲液组分，用于均质化位于损伤中心的脊髓组织以便通过酶联免疫吸附法(ELISA)检测脊髓组织样本中的神经调节素-1 ß1(Nrg-1ß1)

这些 片剂针对常规用途进行了优化和测试。 它们可用于多种应用，包括 和冰冷放射免疫沉淀测试缓冲液一起收集气管残留物 以确定气管基础细胞命运（tracheal basal cell fate），并可使大鼠肺组织均质用于 蛋白免疫印迹分析。

特点和优势

  • 广谱蛋白酶抑制作用：抑制丝氨酸、半胱氨酸、天冬氨酸和金属蛋白酶，这使其成为各种应用中蛋白酶抑制的多功能解决方案。
  • 水溶性片剂形式：易于使用和制备，一片片剂可以制备100mL混合物。
  • 蛋白酶抑制剂混合物：提供全面有效的蛋白酶抑制解决方案。
  • 经过一般用途的测试和优化：经过各种应用验证的高性能和可靠性。

组分

AEBSF，2 mM
抑肽酶，0.3 μM
苯丁抑制素，130 μM
EDTA，1 mM
E-64，14 μM
亮抑酶肽，1 μM

数量

一片可制备100 mL混合物溶液。建议使用1 mL抑制相当于1 mg USP胰酶的蛋白酶。 建议使用1片抑制最多20g细胞提取物中的蛋白酶。

外形

片剂可溶于水。

其他说明

仅供研发使用。&不可用于药物、家庭或其他用途。有关危险和安全处理方法的信息，请参阅安全数据说明书。

法律信息

HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

相关产品

产品编号
说明
价格

P8465

蛋白酶抑制剂混合物粉末, for use with bacterial cell extracts, lyophilized powder

P8340

Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution

P8215

Protease Inhibitor Cocktail, for use with fungal and yeast extracts, DMSO solution

P2714

蛋白酶抑制剂混合物粉末, for general use, lyophilized powder

P8849

Protease Inhibitor Cocktail, for use in purification of Histidine-tagged proteins, DMSO solution

P9599

Protease Inhibitor Cocktail, for plant cell and tissue extracts, DMSO solution

P1860

Protease Inhibitor Cocktail, for use in tissue culture media, DMSO solution

S8830

SIGMAFAST ™蛋白酶抑制剂混合物片剂，无EDTA, for use in purification of Histidine-tagged proteins

象形图

CorrosionExclamation mark
GHS05,GHS07

警示用语：

Danger

危险声明

H314,H335

危险分类

Eye Dam. 1 - Skin Corr. 1A - STOT SE 3

靶器官

Respiratory system

WGK

WGK 3

个人防护装备

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

法规信息

常规特殊物品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the difference between Protease Inhibitors S8820 and P2714?

    S8820 is the tablet version of P2714.  

  6. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with with HIS-Select™ products or any other immobilized metal affinity chromatography (IMAC) products?

    The tablets contain EDTA, a metal chelator, which may remove metal ions from these affinity products. The suggested protease inhibitor for the HIS-Select products is either Product No. S8830 (water soluble tablet) or P8849 (DMSO solution).

  7. Can Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, be used with ELISA?

    Yes, because it is water based.

  8. How should I dissolve Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    You can dissolve the product in 10 ml of water to make a 10X stock solution and then dilute 1:10 for use.

  9. What is the concentration of the inhibitors in Product S8820, SIGMAFAST™ Protease Inhibitor Tablets?

    When reconstituted (one tablet in 100 ml water), concentrations of the component inhibitors are as follows:AEBSF      2 mME-64          14 μMBestatin     130 μMLeupeptin   0.9 μMAprotinin    0.3 μMEDTA        1 mM

  10. How much of Product S8820, SIGMAFAST™ Protease Inhibitor Tablets, needs to be used in cell lysates?

    One tablet is recommended for the inhibition of proteases present in a maximum of 20 grams of cell extract.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Nihar J Mehta et al.
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The dopamine oxidation product cysteinyl-dopamine has attracted attention as a contributor to the death of dopaminergic neurons in Parkinson's disease. Treatment of cysteinyl-dopamine with hypochlorite yields an even more cytotoxic product. This product has potent redox-cycling activity and initiates production
Tran Hong Nha Nguyen et al.
Molecular & cellular proteomics : MCP, 11(11), 1140-1155 (2012-07-31)
Root hairs are single hair-forming cells on roots that function to increase root surface area, enhancing water and nutrient uptake. In leguminous plants, root hairs also play a critical role as the site of infection by symbiotic nitrogen fixing rhizobia
Immunometabolic profiling of T cells from patients with relapsing-remitting multiple sclerosis reveals an impairment in glycolysis and mitochondrial respiration
La Rocca C, et al.
Journal of biological and chemical chronicles, 77, 39-46 (2017)
Giedrius Steponaitis et al.
Oncology letters, 12(5), 3305-3311 (2016-12-03)
Astrocytomas are one of the most common brain tumours; however, the current methods used to characterize these tumours are inadequate. The establishment of molecular markers may identify variables required to improve tumour characterization and subtyping, and may aid to specify
Heather M Brechbuhl et al.
The American journal of pathology, 179(1), 367-379 (2011-06-28)
The purpose of this study was to determine whether β-catenin regulates basal cell fate determination in the mouse trachea. Analysis of TOPGal transgene reporter activity and Wnt/β-catenin pathway gene expression suggested a role for β-catenin in basal cell proliferation and
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