质量水平
形式
lyophilized powder
用途
sufficient for 10-20 large gels (16 × 18 cm)
sufficient for 20-30 mini-gels (10 × 10 cm)
vial sufficient for 50-100 PhastGels
分子量
6,500-200,000 Da
储存温度
2-8°C
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相关类别
应用
SigmaMarkers设计用于Laemmli SDS-PAGE、PhastSystem (Pharmacia) 、以及Schagger和von Jagow 的SDS-PAGE(三甲基甘氨酸)。 SigmaMarkers涵盖大多数蛋白质或其亚基共有的分子量范围。宽、高和低范围分子量标准品与样品缓冲液一起冻干,便于用去离子水溶解后即可使用。该分子量标准品已经过配制,可在电泳和考马斯蓝染色后产生大致相等强度的明确条带分布。
SigmaMarker已被用作十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)中的蛋白质分子量标准。
其他说明
蛋白质(分子量)
抑肽酶,牛肺(6,500)
α -乳清蛋白,牛奶(14,200)
胰蛋白酶抑制剂,大豆(20,000)
胰蛋白酶原,牛胰腺(24,000)
碳酸酐酶,牛红细胞(29,000)
甘油醛-3-磷酸脱氢酶,兔肌肉(36,000)
卵清蛋白,鸡蛋(45,000)
谷氨酸脱氢酶,牛肝(55,000)
白蛋白,牛血清(66,000)
磷酸化酶B,兔肌肉(97,000)
β-半乳糖苷,大肠杆菌 (116000)
肌球蛋白,猪心脏(200,000)
抑肽酶,牛肺(6,500)
α -乳清蛋白,牛奶(14,200)
胰蛋白酶抑制剂,大豆(20,000)
胰蛋白酶原,牛胰腺(24,000)
碳酸酐酶,牛红细胞(29,000)
甘油醛-3-磷酸脱氢酶,兔肌肉(36,000)
卵清蛋白,鸡蛋(45,000)
谷氨酸脱氢酶,牛肝(55,000)
白蛋白,牛血清(66,000)
磷酸化酶B,兔肌肉(97,000)
β-半乳糖苷,大肠杆菌 (116000)
肌球蛋白,猪心脏(200,000)
通过考马斯蓝染色可产生12个蛋白质条带。
重悬
用100 μl去离子水进行溶解,可得到含有2-3.5mg蛋白质/ml的62mM Tris-HCl(pH6.8),1mM EDTA,4%蔗糖,10mM TCEP,2%SDS和0.01%溴酚蓝的溶液。
法律信息
SigmaMarker is a trademark of Sigma-Aldrich Co. LLC
相关产品
产品编号
说明
价格
警示用语:
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
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