形式
lyophilized powder
质量水平
比活
≥1500 units/mg protein
分子量
32.5 kDa
组成
Protein, ≥70% biuret
UniProt登记号
储存温度
−20°C
基因信息
cow ... SOD1(281495)
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一般描述
Research area: Cell signalingSuperoxide Dismutase (SOD), a low molecular weight protein that is found in all aerobic cells of microorganisms, plants, and animals. It exists as three distinct families such as manganese SOD, copper–zinc SOD, and extracellular SOD.
应用
来自牛肝脏的超氧化物歧化酶已被用于一项研究，以确定高胆固醇血症增加内皮超氧阴离子的产生。 来自牛肝脏的超氧化物歧化酶也被用于研究牛红细胞超氧化物歧化酶的重氮偶联、亚基相互作用和电泳变体。
生化/生理作用
催化超氧自由基歧化为过氧化氢和分子氧。在防御细胞对抗氧自由基的毒性作用中起到关键作用。与一氧化氮 (NO) 竞争超氧阴离子（与 NO 反应生成过氧亚硝基阴离子），从而 SOD 促进 NO 的活性。在培养的大鼠卵泡、神经细胞系和转基因小鼠中，SOD 也显示出抑制细胞凋亡的作用。
单位定义
在 25℃、pH 7.8 的黄嘌呤氧化酶偶联系统中，1 个单位将抑制细胞色素 c 减少 50%（反应体积 3.0 mL）。黄嘌呤氧化酶浓度应每分钟 产生 0.025±0.005 初始 ΔA 550 。
外形
含磷酸钾缓冲盐的冻干粉
分析说明
关于试验方法请参阅 McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969)。
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
Bovine erythrocyte superoxide dismutase: diazo coupling, subunit interactions, and electrophoretic variants.
D P Malinowski et al.
Biochemistry, 18(1), 237-244 (1979-01-09)
Characterisation by EPR spectroscopy of the co-ordination environment of copper in superoxide dismutase from horseradish (Armoracia rusticana Gaertn.)
Palivan, CG and Palivan, H
Proceedings of the Royal Society of Edinburgh. Section B: Biology, 102, 273-277 (1994)
Naoya Ichimaru et al.
Biochemistry, 47(40), 10816-10826 (2008-09-11)
The mode of action of Deltalac-acetogenins, strong inhibitors of bovine heart mitochondrial complex I, is different from that of traditional inhibitors such as rotenone and piericidin A [Murai, M., et al. (2007) Biochemistry 46 , 6409-6416]. As further exploration of
Lu Miao et al.
Free radical biology & medicine, 47(4), 344-356 (2009-05-30)
Numerous short-lived and highly reactive oxygen species (ROS) such as superoxide (O2(.-)), hydroxyl radical, and hydrogen peroxide are continuously generated in vivo. Depending upon concentration, location, and intracellular conditions, ROS can cause toxicity or act as signaling molecules. The cellular
S D Yan et al.
The Journal of biological chemistry, 269(13), 9889-9897 (1994-04-01)
Attack by reactive oxygen intermediates, common to many kinds of cell/tissue injury, has been implicated in the development of diabetic and other vascular diseases. Such oxygen-free radicals can be generated by advanced glycation end products (AGEs), which are nonenzymatically glycated
实验方案
在测定超氧化物歧化酶活性时，使用了一种在550 nm处的连续分光光度法进行测定。一个酶单位可将酶偶联系统的细胞色素c还原率抑制50％。
Enzymatic Assay of Superoxide Dismutase
Separation of Superoxide dismutase
Chromatograms
application for HPLC我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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