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一般描述
单体肌氨酸氧化酶（MSOX）是一种黄素酶，可催化肌氨酸（N-甲基甘氨酸）发生氧化去甲基化，产生甘氨酸、甲醛和过氧化氢。单体肌氨酸氧化酶可以氧化其他次级氨基酸，例如N-甲基-L-丙氨酸、N-乙基甘氨酸和L-脯氨酸。
肌氨酸氧化酶是 44kDa 蛋白质，其共价结合 1 mol 黄素腺嘌呤二核苷酸（FAD）。它属于具有结合 FAD 的氨基酸氧化酶家族。
应用
来自芽孢杆菌的肌氨酸氧化酶已被用作阳性对照，用于通过 UV 共价结合黄素腺嘌呤二核苷酸（FAD）检测蛋白质。它还被用于通过与铂电极上的其他酶共固定来制备肌酸酐生物传感器。
肌氨酸氧化酶已用于研究固定化构建的安培生物传感器时作为多酶级联反应的一个组份。肌氨酸氧化酶也被用于研究黄素蛋白对胺的氧化作用。
单位定义
在 pH8.3，37℃ 下，一个单位的酶每分钟从肌氨酸形成 1.0 μmole 甲醛。
外形
没有添加稳定剂
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
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Journal of bacteriology, 190(8), 2690-2699 (2007-10-24)
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