生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
IgG fraction of antiserum
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 126 kDa
种属反应性
human, rat (predicted), mouse (predicted)
技术
western blot: 1:250-1:500 using whole cell extract of HEK293-T expressing SMC6
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... SMC6(79677)
mouse ... Smc6(67241)
rat ... Smc6l1(313961)
一般描述
染色体结构维持蛋白6（SCM6）属于染色体结构维持家族。至少有六个SMC家族成员以特定组合形成三个异二聚体。SCM6基因定位于人染色体2p24.2。在结构上，SCM6在N和C末端具有DNA结合结构域，一个长的卷曲螺旋结构域和一个铰链区。
特异性
抗SMC6（也称为SMC6L1）特异性识别SMC6。
免疫原
对应于人SMC6第766-779个氨基酸的合成肽，通过N-末端的半胱氨酸残基与KLH发生偶联。该序列在小鼠中相差一个氨基酸，在大鼠中相差两个氨基酸。
应用
兔抗SMC6抗体可用于免疫印迹。
生化/生理作用
染色体蛋白6（SCM6）和SMC5的结构维持主要参与酵母和人类对DNA损伤的细胞反应。酵母SMC5/6复合物包含几种非SMC元素（NSE），包括甲磺酸甲酯敏感性蛋白21（MMS21），其磺酰化复合物的组分。SMC5/6复合物被募集到核酸酶诱导的双链断裂（DSBs）。此事件对于将人类SMC1/3内聚复合物募集到DSB至关重要。已知SCM6与乳头瘤病毒E2蛋白相互作用。拓扑异构酶IIα的有丝分裂畸变和异常分布与Smc5和Smc6的水平降低有关。
外形
溶于含15 mM叠氮化钠的0.01 M磷酸盐缓冲盐水（pH 7.4）中。
储存及稳定性
若需连续使用，可在2-8℃下保存最长一个月。若需延长储存时间，可将溶液分装并冷冻。不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。如果在12小时内未使用，则应丢弃工作稀释样品。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
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