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重组
expressed in E. coli
质量水平
检测方案
≥75% (SDS-PAGE)
形式
aqueous glycerol solution
比活
100 units/mg protein (Bradford)
分子量
protein apparent mol wt ~66 kDa
UniProt登记号
运输
dry ice
储存温度
−70°C
基因信息
human ... SGK2(10110)
应用
Kinase activity is measured as the molar amount of phosphate incorporated into the Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P] ATP.
生化/生理作用
SGK2 is a member of the serum- and glucocorticoid-induced kinases (SGK) which are serine-threonine kinases and belong to the "AGC" kinase subfamily, which includes protein kinases A, G, and C and has SGK2 enhances Na+/K+-ATPase activity in a variety of cells. In addition, SGK2 mimics the function of SGK1 and SGK3 and participates in the regulation of renal epithelial Na+ channel ENaC activity.
单位定义
One unit will incorporate one nanomole of phosphate into the AKT/SGK substrate peptide(RPRAATF) per minute at 30 °C and pH 7.2 using a final concentration of 50 μM [32P] ATP.
外形
Supplied at 50 μL in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA, and 30% glycerol.
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
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