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Merck
CN

S5897

Sigma-Aldrich

葡聚糖凝胶 ® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

CAS号:
MDL编号:
UNSPSC代码:
23151817
NACRES:
NA.52

等级

DNA grade
for molecular biology

质量水平

产品线

BioReagent

表单

beads

技术

DNA purification: suitable

基质

cross-linked dextran

溶胀

1 g swells to 9-11 mL

珠子直径

20-80 μm

孔径

exclusion limit (20 bp dsDNA (rA)20)

pH值(酸碱度)

2-10

异质活性

DNase, RNase, none detected

储存温度

room temp

SMILES字符串

O1C(C(C(C(C1CO)O)O)O)OCC2OC(C(C(C2O)O)O)OCC(O)C(O)C(O)C(O)C=O

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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一般描述

塞法戴克斯 ® G-50,Fine 是一种常用的凝胶过滤介质,用于 >30,000 MW 生物分子的脱盐和缓冲液交换。塞法戴克斯®通过葡聚糖与环氧氯丙烷交联制备。塞法戴克斯的交联程度不同,因此其溶胀程度和分子分馏范围也不同。“Fine”表示具有极好的效率和较短的扩散距离的小粒径。

S5897-100G 的更新产品编号为 GE17-0573-02

应用

塞法戴克斯 ® G-50 适用于离心柱纯化 DNA(来自污染的小分子),用于杂交。用于 DNA 测序前纯化 PCR 产物,以提高测序质量。

特点和优势

  • 极佳的回收率
  • 最小样品稀释度
  • 快速且易于使用

法律信息

Sephadex is a registered trademark of Cytiva

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Torben Riehl et al.
PloS one, 7(11), e49354-e49354 (2012-11-13)
The Amundsen Sea, Antarctica, is amongst the most rapidly changing environments of the world. Its benthic inhabitants are barely known and the BIOPEARL 2 project was one of the first to biologically explore this region. Collected during this expedition, Macrostylis
Aron Fazekas et al.
BioTechniques, 48(4), 277-285 (2010-06-24)
Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
Mark Del Campo et al.
Journal of molecular biology, 389(4), 674-693 (2009-04-28)
The DEAD-box proteins CYT-19 in Neurospora crassa and Mss116p in Saccharomyces cerevisiae are broadly acting RNA chaperones that function in mitochondria to stimulate group I and group II intron splicing and to activate mRNA translation. Previous studies showed that the
Ryo Takeuchi et al.
Nucleic acids research, 37(3), 877-890 (2008-12-24)
The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve
Katharina Besemer et al.
Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the

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