SYPRO^® Red Protein Gel Stain

SYPRO Red is similar to SYPRO Orange although it gives slightly lower background fluorescence and is not quite as bright as SYPRO Orange. For those using a laser-excited gel scanner, a He-Ne or Nd:YAG laser-based instrument is recommended for SYPRO Red. The dye is efficiently excited by UV or broad band illumination. It is not suitable for staining proteins on blots or in IEF gels and shows reduced sensitivity when staining proteins on 2-D gels.
SYPRO Red is:

• Highly sensitive. The stain can detect 1 to 2 ng of protein per mini gel band, making it more sensitive than Coomassie Brilliant Blue or silver staining.
• Rapid. Staining is complete in less than one hour.
• Simple. After electrophoresis, the gel is stained, rinsed and photographed; no fixation or destaining steps are required.
• Compatible with standard laboratory equipment. Stained proteins can be visualized with a standard 300 nm UV transilluminator or laser scanner.
• Cost-effective. Staining with SYPRO Red is less expensive than silver staining and requires less time.
• Low protein-to-protein variability. The dye interacts with the SDS coat around the protein, giving more consistent staining between different types of proteins compared to Coomassie or silver staining.
• Selective for proteins. SYPRO Red detects proteins as small as 6.5 kDa and does not stain nucleic acids or lipopolysaccharides. It does stain glycosylated proteins..
• Broad linear range of detection. The fluorescence intensity of the stained bands is linear with protein quantity over three orders of magnitude (a much broader range than either Coomassie or silver staining).

Store desiccated and protect from light.

SYPRO is a registered trademark of Life Technologies