Anti-SUMO-1 (C-terminal) antibody produced in rabbit

Small ubiquitin-related modifier 1 (SUMO-1) is a highly conserved small ubiquitin-related modifier, also known as sentrin. SUMO-1 gene is mapped to human chromosome 2q33.1.

Anti-SUMO-1 (C-terminal) recognizes unconjugated SUMO-1 (14 kDa), SUMO-1-fusion protein, as well as proteins covalently conjugated to SUMO-1, eg. RanGAP1, 90kDa

synthetic peptide corresponding to amino acids 86-97 located at the C-terminus of human SUMO-1, conjugated to KLH. This sequence is identical in many species including rat, mouse, bovine, chicken, and Xenopus, and highly conserved (single amino acid substitution) in C. elegans SMT3. The sequence has only 66% homology to human SUMO-2 and SUMO-3.

Anti-SUMO-1 (C-terminal) antibody produced in rabbit has been used in immunoblotting.

Small ubiquitin-related modifier 1 (SUMO-1) is covalently conjugated to the lysine side in proteins through its C-terminal glycine residue by an isopeptide bond. Their conjugation with cellular proteins has been correlated wide variety of biological processes including inflammation, oncogenesis nuclear transport, cell cycle control and viral infection response. SUMO-1 serves as a substrate for Ran GTPase-activating protein (RanGAP1). Unmodified RanGAP1 is present in the cytoplasm, suggesting that modification by SUMO-1 may target RanGAP1 to the nuclear pore complex (NPC). Haploinsufficiency in the SUMO-1 gene is implicated in the Cleft Lip and Palate.

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

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